Gene

KWMTBOMO00380  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA000511

Annotation

3-hydroxyacyl-CoA_dehydrogenase_[Bombyx_mori]

Location

Bomo_Chr1(-):11274584-11280808      View in EpiBrowser

Full name

3-hydroxyacyl-CoA dehydrogenase type-2      

Alternative Name

17-beta-hydroxysteroid dehydrogenase 10
3-hydroxy-2-methylbutyryl-CoA dehydrogenase
3-hydroxyacyl-CoA dehydrogenase type II
Mitochondrial ribonuclease P protein 2
Scully protein
Type II HADH

Location in the cell

Cytoplasmic   Reliability : 1.04 Mitochondrial   Reliability : 1.392

CDS

ATGTTTAAGGGCCTAGTAGCTTTGGTGACTGGAGGGTCATCAGGATTAGGAAAAGCTACTGCTGAGCTGTTAGTAAAACAAGGGGGCAGAGTTGTTATCTGTGACTTACCCTCAACCAAAGGCCAAAATACTGCTAAAAGCCTTGGCGAGAATGCAGTGTTTGCACCTGTTGATGTAACTTCGGAAGATGATGTGAAGGAGGCTCTACAGATAACTTTAGACAAGTTTGGTCGATTGGATGCTCTTATAAACTGTGCAGGCGTTGCGAGTGCATCAAGAGTTTATAATTTCAAAAAGGAACAACCATTTGATTTGAACATCTTTCAAAACACTATTCAGGTAAATTTGATAGGAACATTCAATGTTATTCGCTTAGCAGTGGGGCTAATCGGGAAAAATGCACCAGACGCTGACGGACAACGCGGAGTCGTTATCAACACGGCCAGTGTCGCTGCATTTGATGGACAGATCGGACAGGCAGCTTATTCTGCTTCCAAAGCGGGCGTGGTAGGCATGACATTGCCTCTTGCAAGGGACATGGCCAAACAAGGAATCAGAGTCGTCACTATTGCACCAGGATTATTCCGTACACCTATGATGGAACAATTGCCAGAGCCGGCGATCAAAAGCCTGGAAGCGTCGGTGCCATTCCCTTCTAGACTTGGGCATCCTCAAGAGTTTGCATTGCTCGTTCAGAGCATCATTCAGAACCCGATGCTAAACGGCGAAACTATACGATTAGACGGCTCCCTCAGGATGCAGCCTTAA

Protein

MFKGLVALVTGGSSGLGKATAELLVKQGGRVVICDLPSTKGQNTAKSLGENAVFAPVDVTSEDDVKEALQITLDKFGRLDALINCAGVASASRVYNFKKEQPFDLNIFQNTIQVNLIGTFNVIRLAVGLIGKNAPDADGQRGVVINTASVAAFDGQIGQAAYSASKAGVVGMTLPLARDMAKQGIRVVTIAPGLFRTPMMEQLPEPAIKSLEASVPFPSRLGHPQEFALLVQSIIQNPMLNGETIRLDGSLRMQP

Description

May function in mitochondrial tRNA maturation. Catalyzes the beta-oxidation at position 17 of androgens and estrogens, and has 3-alpha-hydroxysteroid dehydrogenase activity with androsterone. Catalyzes the third step in the beta-oxidation of fatty acids. Carries out oxidative conversions of 7-beta-hydroxylated bile acids. Also exhibits 20-beta-OH and 21-OH dehydrogenase activities with C21 steroids. Required for cell survival during embryonic development. May play a role in germline formation.

Catalytic Activity

a (3S)-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) + NADH
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD(+) = 2-methyl-3-oxobutanoyl-CoA + H(+) + NADH
NAD(+) + testosterone = androst-4-ene-3,17-dione + H(+) + NADH
NADP(+) + testosterone = androst-4-ene-3,17-dione + H(+) + NADPH

Biophysicochemical Properties

33.7 uM for acetoacetyl-CoA (in the presence of 0.2 mM NADH, at pH 6.4 and 25 degrees Celsius)
101 uM for beta-hydroxybutyryl-CoA (in the presence of 1 mM NAD, at pH 9.3 and 25 degrees Celsius)
37.3 uM for androsterone (in the presence of 1 mM NAD, at pH 9.3 and 25 degrees Celsius)
12.3 uM for 5-alpha-dihydrotestosterone (in the presence of 0.2 mM NADH, at pH 6.4 and 25 degrees Celsius)
11.1 uM for 17-beta-estradiol (in the presence of 0.2 mM NADH, at pH 9.3 and 25 degrees Celsius)
9 uM for 5-alpha-pregnan-20-beta-ol-3-one (in the presence of 1 mM NAD, at pH 9.3 and 25 degrees Celsius)
3 uM for isoursodeoxycholic acid (in the presence of 1 mM NAD, at pH 9.3 and 25 degrees Celsius)
32.5 uM for NADH (in the presence of acetoacetyl-CoA, at pH 7.0 and 25 degrees Celsius)
64.4 uM for NAD (in the presence of beta-hydroxybutyryl-CoA, at pH 9.3 and 25 degrees Celsius)
124 uM for NAD (in the presence of aldosterone, at pH 9.3 and 25 degrees Celsius)

Subunit

Multimer.

Similarity

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Keywords

Complete proteome   Mitochondrion   NAD   Oxidoreductase   Reference proteome   tRNA processing  

Feature

chain  3-hydroxyacyl-CoA dehydrogenase type-2

Uniprot

Q1HPX9   A0A2H1W0W2   A0A068FJH4   A0A194PLH7   A0A194QL99   S4PGS4   + More
A0A212EGK3   A0A1W4XJI9   A0A2C9JID1   Q4PP87   D6WFE2   A0A1Y1LRU1   D3TLT8   A0A1A9XA65   B4M6R1   A0A0L8HI08   E9FRN7   B4L296   N6UI45   A0A1W4XN13   T1PE05   R4V243   B4JNB1   A0A0M4EZZ4   E2A478   A0A1I8P3Q2   V9LAI2   A0A1I8P3Q5   H3B6K3   A0A1B0GJR4   A0A0A9Z6D1   Q28CR9   B3MXV0   B4Q2N6   B3NTA4   A9ULK6   A0A336LPQ3   A0A0P4VTY5   R4FNB7   A0A034WG91   A0A0C9R451   A0A067R1F2   A0A3P8VIE9   A0A3B3C5E4   A0A0K8UAH4   A0A1B6LJU8   A0A165A378   A0A1W4UN75   A0A1W4XXI0   A0A3B3HLN0   A0A3P9IJ16   A0A1D2M829   O18404   B4I6N3   F7DFF3   W8C108   V3ZRR5   A0A3B3ZWX2   A0A1B6JZI7   U3GM45   Q6DCM8   A0A1L8DZJ3   A0A3P8VNN4   A0A0P4X8P7   B4NEB5   A0A0A1X8C9   A0A3B4WH98   A0A3B4TR38   E3TDR7   A0A3B4WFP0   A0A3B4TR48   A0A1A7XM58   A0A146NWF8   A0A0S7HKX2   A0A182W0K4   A0A1B6F172   A0A069DY28   A0A3B1KFP3   A0A023F9Q1   A0A0K8RNJ1   A0A0J7L9M1   A0A2R5LN65   A0A182R810   M7AQR3   A0A1B6D4D0   A0A182Y4I3   A0A0V0G444   A0A1L8F1N3   G1NSP2   A0A224XV14   E3TDJ4   A0A293MXH6   A0A0P4ZN24   A0A3B5QP52   B5XGY1   A0A3B4C668   Q5XJS8   A0A195FUW0   A0A2D0SH21   A0A0F8ASV8  

EC Number

1.1.1.35

Pubmed

19121390    26385554    26354079    23622113    22118469    15562597    + More
18362917    19820115    28004739    20353571    17994087    21292972    23537049    25315136    20798317    24402279    9215903    25401762    26823975    17550304    27129103    25348373    24845553    24487278    29451363    17554307    27289101    9585418    10731132    12537572    12537569    12917011    16979555    17924685    20431018    24495485    23254933    25463417    25830018    20634964    26334808    25329095    25474469    23624526    25244985    27762356    21993624    23542700    20433749    23594743    25835551   

EMBL

BABH01007178    DQ443273    ABF51362.1    ODYU01005657    SOQ46731.1    KJ622102    + More
AID66692.1    KQ459605    KPI91960.1    KQ461196    KPJ06338.1    GAIX01006085    JAA86475.1    AGBW02015069    OWR40612.1    DQ062226    AAY63981.1    KQ971319    EFA00491.1    GEZM01050546    GEZM01050545    JAV75558.1    CCAG010012474    EZ422390    ADD18666.1    CH940653    EDW62478.1    KQ418094    KOF88861.1    GL732523    EFX89885.1    CH933810    EDW07757.1    APGK01019219    KB740096    ENN81415.1    KA646375    AFP61004.1    KC740963    AGM32787.1    CH916371    EDV92204.1    CP012528    ALC49867.1    GL436596    EFN71749.1    JW876647    AFP09164.1    AFYH01083165    AFYH01083166    AFYH01083167    AFYH01083168    AFYH01083169    AFYH01083170    AFYH01083171    AJWK01022146    GBHO01003590    GBRD01011971    GBRD01011970    GDHC01009188    JAG40014.1    JAG53853.1    JAQ09441.1    CR926229    CAJ81399.1    CH902630    EDV38565.1    CM000162    EDX02677.1    CH954180    EDV46555.1    BC157293    AAI57294.1    UFQT01000107    SSX20064.1    GDKW01000596    JAI55999.1    ACPB03014675    GAHY01000628    JAA76882.1    GAKP01005605    JAC53347.1    GBYB01001591    JAG71358.1    KK852996    KDR12703.1    GDHF01028999    JAI23315.1    GEBQ01016088    JAT23889.1    LRGB01000642    KZS17123.1    LJIJ01002921    ODM89092.1    Y15102    AE014298    AY121672    BT029045    BT132763    CH480823    EDW55981.1    AAMC01047154    AAMC01047155    GAMC01003782    JAC02774.1    KB203301    ESO85245.1    GECU01003373    JAT04334.1    KF031120    AGT97387.1    BC077977    AAH77977.1    GFDF01002382    JAV11702.1    GDIP01244853    JAI78548.1    CH964239    EDW82084.1    GBXI01007369    JAD06923.1    GU588497    ADO28453.1    HADW01017781    HADX01009706    SBP19181.1    GCES01150525    JAQ35797.1    GBYX01439786    JAO41572.1    GECZ01025777    JAS43992.1    GBGD01002615    JAC86274.1    GBBI01001059    JAC17653.1    GADI01001659    JAA72149.1    LBMM01000187    KMR04548.1    GGLE01006818    MBY10944.1    KB575110    EMP26949.1    GEDC01016805    JAS20493.1    GECL01003313    JAP02811.1    CM004481    OCT65485.1    AAPE02031626    GFTR01004176    JAW12250.1    GU588424    ADO28380.1    GFWV01019322    MAA44050.1    GDIP01212395    JAJ11007.1    BT050300    BT050344    ACI70101.1    ACI70145.1    CR735130    BC083219    BC165246    AAH83219.1    AAI65246.1    KQ981261    KYN44077.1    KQ040919    KKF33011.1   

Proteomes

UP000005204    UP000053268    UP000053240    UP000007151    UP000192223    UP000076420    + More
UP000007266    UP000092444    UP000092443    UP000008792    UP000053454    UP000000305    UP000009192    UP000019118    UP000192224    UP000095301    UP000001070    UP000092553    UP000000311    UP000095300    UP000008672    UP000092461    UP000007801    UP000002282    UP000008711    UP000015103    UP000027135    UP000265120    UP000261560    UP000076858    UP000192221    UP000001038    UP000265180    UP000265200    UP000094527    UP000000803    UP000001292    UP000008143    UP000030746    UP000261520    UP000007798    UP000261360    UP000261420    UP000221080    UP000075920    UP000018467    UP000036403    UP000075900    UP000031443    UP000076408    UP000186698    UP000001074    UP000002852    UP000087266    UP000261440    UP000000437    UP000078541   

PRIDE

Q1HPX9    O18404   

Pfam

PF00106   adh_short

Interpro

IPR002347   SDR_fam        + More
IPR020904   Sc_DH/Rdtase_CS       
IPR036291   NAD(P)-bd_dom_sf       

SUPFAM

SSF51735   SSF51735       

ProteinModelPortal

Q1HPX9   A0A2H1W0W2   A0A068FJH4   A0A194PLH7   A0A194QL99   S4PGS4   + More
A0A212EGK3   A0A1W4XJI9   A0A2C9JID1   Q4PP87   D6WFE2   A0A1Y1LRU1   D3TLT8   A0A1A9XA65   B4M6R1   A0A0L8HI08   E9FRN7   B4L296   N6UI45   A0A1W4XN13   T1PE05   R4V243   B4JNB1   A0A0M4EZZ4   E2A478   A0A1I8P3Q2   V9LAI2   A0A1I8P3Q5   H3B6K3   A0A1B0GJR4   A0A0A9Z6D1   Q28CR9   B3MXV0   B4Q2N6   B3NTA4   A9ULK6   A0A336LPQ3   A0A0P4VTY5   R4FNB7   A0A034WG91   A0A0C9R451   A0A067R1F2   A0A3P8VIE9   A0A3B3C5E4   A0A0K8UAH4   A0A1B6LJU8   A0A165A378   A0A1W4UN75   A0A1W4XXI0   A0A3B3HLN0   A0A3P9IJ16   A0A1D2M829   O18404   B4I6N3   F7DFF3   W8C108   V3ZRR5   A0A3B3ZWX2   A0A1B6JZI7   U3GM45   Q6DCM8   A0A1L8DZJ3   A0A3P8VNN4   A0A0P4X8P7   B4NEB5   A0A0A1X8C9   A0A3B4WH98   A0A3B4TR38   E3TDR7   A0A3B4WFP0   A0A3B4TR48   A0A1A7XM58   A0A146NWF8   A0A0S7HKX2   A0A182W0K4   A0A1B6F172   A0A069DY28   A0A3B1KFP3   A0A023F9Q1   A0A0K8RNJ1   A0A0J7L9M1   A0A2R5LN65   A0A182R810   M7AQR3   A0A1B6D4D0   A0A182Y4I3   A0A0V0G444   A0A1L8F1N3   G1NSP2   A0A224XV14   E3TDJ4   A0A293MXH6   A0A0P4ZN24   A0A3B5QP52   B5XGY1   A0A3B4C668   Q5XJS8   A0A195FUW0   A0A2D0SH21   A0A0F8ASV8  

PDB

2O23     E-value=9.19295e-85,     Score=797

KEGG

732961    K08683 3-hydroxyacyl-CoA dehydrogenase / 3-hydroxy-2-methylbutyryl-CoA dehydrogenase [EC:1.1.1.35 1.

PATHWAY

00280    Valine, leucine and isoleucine degradation - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0016491   GO:0030678   GO:0018454   GO:0006631   GO:0047035   GO:0047022   GO:0004303   GO:0090646   GO:0008209   GO:0006637   GO:0008205   GO:0008210   GO:0047015   GO:0090502   GO:0005739   GO:0005829   GO:0016229   GO:0008202   GO:0003857   GO:0030283   GO:0035410   GO:0016020   GO:0009408   GO:0009409   GO:0005634   GO:0006355   GO:0008270   GO:0043565   GO:0043401   GO:0003677   GO:0003707   GO:0007169   GO:0016021  

Subcellular location

Mitochondrion   Localizes to the lipid droplet fraction in early embryos.   With evidence from 4 publications.

Length:

255

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.11294

Exp number, first 60 AAs:

0.01566

Total prob of N-in:

0.01617

outside

1  -  255

 

 

Pi

2.092111

Theta

6.502945

Tajima's D

-2.038565

CLR

0.090448

CSRT

0.00919954002299885

Interpretation

Uncertain