Gene

KWMTBOMO00347  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA000704

Annotation

PREDICTED:_procollagen-lysine?2-oxoglutarate_5-dioxygenase_1_isoform_X1_[Bombyx_mori]

Location

Bomo_Chr1(+):10420439-10427409      View in EpiBrowser

Full name

Procollagen-lysine,2-oxoglutarate 5-dioxygenase      

Alternative Name

Procollagen lysyl hydroxylase

Location in the cell

Extracellular   Reliability : 1.292 Nuclear   Reliability : 1.223

CDS

ATGTCGCTGTTCAGAGCGCCTAAGGCCAAAGCTGTGACTTATGTACAGGAAGGACACGACTCTGACATGGCATTCTGCGCGAGTCTCAGAGAGCTCAATATATTCATGTATGTGAGCAACGAAGAGGATTTCGGCCATCTCGTTAATCCGGAAACATATGACATAACAAAAACCCATCCCGATATGTACCAGCTCTTCGAGAATAATATGGAATGGACTACCCGTTATCTGCACCCTGAATACCTGGCTAACTTCGAAGAGGATCGGAAACATTTAATGCCGTGCACTGATGTTTATTGGTTCCCCTTGATGTCTGATAGATTCTGTGATGAGTGGATCGCTATAATGGAAGCATACGGAAAATGGAGCGATGGAACCAATAATGACAAACGTTTGGAAAGCGGCTACGAAGCTGTGCCCACTCGAGATATCCACATGAGCCAAGTCGGCTTGGAACGTCATTGGCTACAGATCCTGAAGGATTACGTACGACCACTTCAAGAATTAGTCTTCACCGGATACTATCATAATCCTCCAGCGTCTATAATGAATTTTGTTGTTCGTTACCGACCGGATGAACAACCATCGCTGCGACCTCATCACGATTCCTCTACATATACCATCAACTTGGCTCTTAACACTCCTAACGTGGACTACGAAGGCGGTGGCTGCAGATTCATCAGGTATAATTGTTCGGTTCGCAACACTAAGAAAGGCTGGCTCCTCATGCATCCGGGACGTCTAACTCACTACCACGAGGGTCTCCTCGTCACCAAAGGAACTCGATACATCATGATTTCCTTCGTGGATCCATGA

Protein

MSLFRAPKAKAVTYVQEGHDSDMAFCASLRELNIFMYVSNEEDFGHLVNPETYDITKTHPDMYQLFENNMEWTTRYLHPEYLANFEEDRKHLMPCTDVYWFPLMSDRFCDEWIAIMEAYGKWSDGTNNDKRLESGYEAVPTRDIHMSQVGLERHWLQILKDYVRPLQELVFTGYYHNPPASIMNFVVRYRPDEQPSLRPHHDSSTYTINLALNTPNVDYEGGGCRFIRYNCSVRNTKKGWLLMHPGRLTHYHEGLLVTKGTRYIMISFVDP

Description

Forms hydroxylysine residues in collagen type IV (By similarity). Required for the secretion of collagen type IV (vkg) from haemocytes, fat body and follicle cells (PubMed:23369713, PubMed:20888931).

Catalytic Activity

2-oxoglutarate + L-lysyl-[procollagen] + O2 = (5R)-5-hydroxy-L-lysyl-[procollagen] + CO2 + succinate

Cofactor

L-ascorbate
Fe(2+)

Keywords

Complete proteome   Dioxygenase   Endoplasmic reticulum   Glycoprotein   Iron   Metal-binding   Oxidoreductase   Reference proteome   Secreted   Signal   Vitamin C  

Feature

chain  Procollagen-lysine,2-oxoglutarate 5-dioxygenase

Uniprot

H9ITX4   A0A2H1W0V4   A0A2A4IV70   A0A194QUV5   A0A212F445   A0A194PRJ2   + More
S4NHY6   E0VRF9   D6WLT6   A0A0T6B0C6   A0A1Y1JYY2   A0A2J7RR28   A0A0J7L7I5   N6U6J7   A0A2J7RR31   W8CBA7   A0A3L8DFR0   A0A026WU85   T1IVE2   K7IVX4   Q9VTH0   A0A1W4W5Y7   A0A1L8E427   B3NGT1   A0A1W4WHP5   B4QPR5   B4HEB5   A0A158NHE9   A0A3B0J552   A0A1I8M2M7   T1PHD0   U4UGS4   B4PF19   A0A336MPH5   A0A3B0J9K2   A0A1B6D872   U5ESN7   A0A182M555   A0A2M4AJ24   N6U9E2   A0A2M4BFG4   A0A2Y9D3K2   A0A2H8TSM2   A0A2M4BFC2   W8BR58   A0A161MQ37   A0A2S2PZX1   A0A182R4Z2   A0A182GDY8   A0A182YB77   A0A182U4L3   A0A1B6F2J3   A0A1B6GRF9   A0A2C9GQL5   A0A146LKA1   Q7PXP4   A0A146L4I6   A0A182L9E9   A0A1S4FIM3   Q0IER9   W5J8M0   A0A182UT40   B3M3Y1   A0A1I8JSV0   A0A0C9PND8   A0A182PGY1   B4LC63   A0A1I8NP49   A0A0K8UXR4   Q2LZ43   B4L188   A0A034VR52   A0A1W0WF74   A0A023EXP1   A0A1B0CUR7   A0A336KYV9   A0A2S2NP37   B4J2V7   A0A0A1WXZ2   A0A1A9W4M0   A0A1B6LRI1   A0A182QSJ3   A0A182JTM5   A0A293M6J3   T1I002   A0A0P4VSX2   B0WXT7   B4N5C6   A0A293MF06   A0A0M5J3P5   A0A182VWF4   A0A0L0C2W1   A0A2T7PTX1   A0A1D1V9W1   A0A084W539   A0A1Q3FMH4   A0A1W4W7U1   A0A1Z5LFT1   A0A224XJ30   A0A182JHX2  

Pubmed

19121390    26354079    22118469    23622113    20566863    18362917    + More
19820115    28004739    23537049    24495485    30249741    24508170    20075255    10731132    12537572    12537569    20888931    23369713    17994087    22936249    21347285    25315136    17550304    26483478    25244985    26823975    12364791    14747013    17210077    20966253    17510324    20920257    23761445    15632085    25348373    24945155    25830018    27129103    26108605    27649274    24438588    28528879   

EMBL

BABH01007118    ODYU01005652    SOQ46721.1    NWSH01006243    PCG63559.1    KQ461108    + More
KPJ09313.1    AGBW02010446    OWR48492.1    KQ459594    KPI96066.1    GAIX01014234    JAA78326.1    AAZO01004657    DS235465    EEB15965.1    KQ971343    EFA03403.2    LJIG01016333    KRT80915.1    GEZM01100150    JAV52990.1    NEVH01000611    PNF43290.1    LBMM01000375    KMQ98949.1    APGK01037210    APGK01037211    APGK01037212    APGK01037213    KB740948    ENN77265.1    PNF43292.1    GAMC01005001    GAMC01004999    JAC01555.1    QOIP01000008    RLU19234.1    KK107109    EZA59211.1    JH431581    AE014296    BT003764    AY118559    AAM49928.1    AAO41443.1    GFDF01000700    JAV13384.1    CH954178    EDV51388.1    CM000363    CM002912    EDX10056.1    KMY98953.1    CH480815    EDW41069.1    ADTU01015730    OUUW01000002    SPP77044.1    KA647298    AFP61927.1    KB632348    ERL93179.1    CM000159    EDW94101.1    KRK01654.1    UFQT01001734    SSX31565.1    SPP77043.1    GEDC01015436    JAS21862.1    GANO01002271    JAB57600.1    AXCM01000787    GGFK01007463    MBW40784.1    ENN77266.1    GGFJ01002592    MBW51733.1    GFXV01004787    MBW16592.1    GGFJ01002591    MBW51732.1    GAMC01005000    GAMC01004998    JAC01558.1    GEMB01002664    GEMB01002663    JAS00529.1    GGMS01001854    MBY71057.1    JXUM01056878    KQ561930    KXJ77131.1    GECZ01025277    JAS44492.1    GECZ01004755    JAS65014.1    APCN01000879    GDHC01010106    JAQ08523.1    AAAB01008987    EAA00870.4    GDHC01016074    JAQ02555.1    CH477486    EAT40135.1    ADMH02001952    ETN60311.1    CH902618    EDV39315.2    GBYB01002658    JAG72425.1    CH940647    EDW70891.1    GDHF01020855    JAI31459.1    CH379069    EAL29666.3    CH933809    EDW19270.1    GAKP01014909    GAKP01014908    JAC44043.1    MTYJ01000115    OQV13854.1    GAPW01000344    JAC13254.1    AJWK01029612    UFQS01001121    UFQT01001121    SSX09105.1    SSX29016.1    GGMR01006278    MBY18897.1    CH916366    EDV97127.1    GBXI01010932    JAD03360.1    GEBQ01013695    JAT26282.1    AXCN02001525    GFWV01009846    MAA34575.1    ACPB03000862    GDKW01000998    JAI55597.1    DS232173    EDS36747.1    CH964101    EDW79565.1    GFWV01013809    MAA38538.1    CP012525    ALC43711.1    JRES01001072    KNC25754.1    PZQS01000002    PVD36874.1    BDGG01000004    GAU97710.1    ATLV01020474    ATLV01020475    ATLV01020476    KE525302    KFB45333.1    GFDL01006300    JAV28745.1    GFJQ02000717    JAW06253.1    GFTR01007834    JAW08592.1   

Proteomes

UP000005204    UP000218220    UP000053240    UP000007151    UP000053268    UP000009046    + More
UP000007266    UP000235965    UP000036403    UP000019118    UP000279307    UP000053097    UP000002358    UP000000803    UP000192223    UP000008711    UP000000304    UP000001292    UP000005205    UP000268350    UP000095301    UP000030742    UP000002282    UP000075883    UP000076407    UP000075900    UP000069940    UP000249989    UP000076408    UP000075902    UP000075840    UP000007062    UP000075882    UP000008820    UP000000673    UP000075903    UP000007801    UP000069272    UP000075885    UP000008792    UP000095300    UP000001819    UP000009192    UP000092461    UP000001070    UP000091820    UP000075886    UP000075881    UP000015103    UP000002320    UP000007798    UP000092553    UP000075920    UP000037069    UP000245119    UP000186922    UP000030765    UP000192221    UP000075880   

PRIDE

H9ITX4    Q9VTH0   

Pfam

PF03171   2OG-FeII_Oxy        + More
PF00096   zf-C2H2
PF07776   zf-AD

Interpro

IPR005123   Oxoglu/Fe-dep_dioxygenase        + More
IPR006620   Pro_4_hyd_alph       
IPR029044   Nucleotide-diphossugar_trans       
IPR001006   Procol_lys_dOase       
IPR012934   Znf_AD       
IPR013087   Znf_C2H2_type       
IPR036236   Znf_C2H2_sf       

SUPFAM

SSF53448   SSF53448        + More
SSF57667   SSF57667       

Gene 3D

3.90.550.10    

ProteinModelPortal

H9ITX4   A0A2H1W0V4   A0A2A4IV70   A0A194QUV5   A0A212F445   A0A194PRJ2   + More
S4NHY6   E0VRF9   D6WLT6   A0A0T6B0C6   A0A1Y1JYY2   A0A2J7RR28   A0A0J7L7I5   N6U6J7   A0A2J7RR31   W8CBA7   A0A3L8DFR0   A0A026WU85   T1IVE2   K7IVX4   Q9VTH0   A0A1W4W5Y7   A0A1L8E427   B3NGT1   A0A1W4WHP5   B4QPR5   B4HEB5   A0A158NHE9   A0A3B0J552   A0A1I8M2M7   T1PHD0   U4UGS4   B4PF19   A0A336MPH5   A0A3B0J9K2   A0A1B6D872   U5ESN7   A0A182M555   A0A2M4AJ24   N6U9E2   A0A2M4BFG4   A0A2Y9D3K2   A0A2H8TSM2   A0A2M4BFC2   W8BR58   A0A161MQ37   A0A2S2PZX1   A0A182R4Z2   A0A182GDY8   A0A182YB77   A0A182U4L3   A0A1B6F2J3   A0A1B6GRF9   A0A2C9GQL5   A0A146LKA1   Q7PXP4   A0A146L4I6   A0A182L9E9   A0A1S4FIM3   Q0IER9   W5J8M0   A0A182UT40   B3M3Y1   A0A1I8JSV0   A0A0C9PND8   A0A182PGY1   B4LC63   A0A1I8NP49   A0A0K8UXR4   Q2LZ43   B4L188   A0A034VR52   A0A1W0WF74   A0A023EXP1   A0A1B0CUR7   A0A336KYV9   A0A2S2NP37   B4J2V7   A0A0A1WXZ2   A0A1A9W4M0   A0A1B6LRI1   A0A182QSJ3   A0A182JTM5   A0A293M6J3   T1I002   A0A0P4VSX2   B0WXT7   B4N5C6   A0A293MF06   A0A0M5J3P5   A0A182VWF4   A0A0L0C2W1   A0A2T7PTX1   A0A1D1V9W1   A0A084W539   A0A1Q3FMH4   A0A1W4W7U1   A0A1Z5LFT1   A0A224XJ30   A0A182JHX2  

PDB

6FXY     E-value=9.07816e-90,     Score=840

KEGG

101743038    K13647 procollagen-lysine,2-oxoglutarate 5-dioxygenase, invertebrate [EC:1.14.11.4] | (RefSeq) proco

PATHWAY

00310    Lysine degradation - Bombyx mori (domestic silkworm)

GO

GO:0005506   GO:0016705   GO:0031418   GO:0051213   GO:0008475   GO:0046947   GO:0030199   GO:0032963   GO:0017185   GO:0005783   GO:0005615   GO:0033823   GO:0048471   GO:0007295   GO:0050211   GO:0008270   GO:0003676   GO:0005634   GO:0016021   GO:0005581   GO:0016491   GO:0030286   GO:0006813   GO:0006810   GO:0009072   GO:0007169   GO:0055114  

Subcellular location

Endoplasmic reticulum   Detected in the ER of fat body cells and haemocytes. Also detected in small puncta in the extracellular space, particularly in the region surrounding the haemocytes.   With evidence from 6 publications.
Secreted   Detected in the ER of fat body cells and haemocytes. Also detected in small puncta in the extracellular space, particularly in the region surrounding the haemocytes.   With evidence from 6 publications.
Extracellular space   Detected in the ER of fat body cells and haemocytes. Also detected in small puncta in the extracellular space, particularly in the region surrounding the haemocytes.   With evidence from 6 publications.

Length:

271

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0039

Exp number, first 60 AAs:

0

Total prob of N-in:

0.03530

outside

1  -  271

 

 

Pi

7.440656

Theta

21.888376

Tajima's D

-1.449111

CLR

0.141342

CSRT

0.063946802659867

Interpretation

Possibly Positive selection