Gene

KWMTBOMO00293  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA000680

Annotation

PREDICTED:_glutamine_synthetase_2_cytoplasmic_[Papilio_machaon]

Location

Bomo_Chr1(+):9305668-9318346      View in EpiBrowser

Full name

Glutamine synthetase       + More
Glutamine synthetase 2 cytoplasmic      

Alternative Name

Glutamate--ammonia ligase 2

Location in the cell

Cytoplasmic   Reliability : 2.131

CDS

ATGGCTGATACAGCGCATACCAAAATAGAAGATAATCCGAAGATTCTATCGGGGCCGGTTTTAACCAACTCCCCGAACGCAGTGCTGTCCAAAACGCTACTGAGTCGATACAATGATCTTCCACTGCCGGCTGACAAGATTCTCGCCACCTACATTTGGATCGACGGCTCTGGCGAACACCTGAGGTGCAAAGATCGCACCTTGAATTTCATACCTAAAGCTCCCAAAGATCTGCCTATATGGAACTTCGATGGCAGCTCAACCAACCAAGCTGATGGGCACAATTCTGATACCTACCTCGTACCTCGTGCTATTTACAAGGATCCATTCCGTCGAGGAAATCACATCCTCGTTATGTGTGATACGTATAAATACAACATGGAGCCAACAGAGAGCAACAATCGCATCAGTTGTCAAGAAGCTTACGACAAATGCAAGGATGATGAGCCCTGGTTTGGGATAGAACAGGAATATATTTTACTGGATTCAGATCTGCGCCCCTTCGGGTGGCCCCCGGGTGGTTTCCCCCCACCGCAAGGGCCATACTACTGCGGTGTCGGTGCCAACAAAGTCTTTGCCAGAGACCTTGTCGAAGCCCACTATCGATGCTGTCTTTATGCTGGCGTACCGATTGCCGGTACAAACGCTGAAGTGATGCCTTCACAGTGGGAGTTCCAAGTCGGTCCCTCAATTGGAGTGCATGCTGGTGATGACCTTTGGGTCGCTCGTTACATTTTGCACAGACTAGCTGAAGAATACGGTGTTATTGTTACGTTTGATCCTAAGCCCGTTCAAGATTGGAATGGATCTGGCGCTCACACCAACTTCTCTACAAAGAAGATGAGAAATGATAACGGTATCATTGAAATCGAAAAAGCGATTGATAAACTCTCCAAAGTGCATATGAAGCACATTAAGGTTTATGATCCTCGCGGTGGAAAAGATAATGAGAGAAGGCTGACTGGTCTTCATGAAACTGCCAGTATTAATGACTTCAGTGCAGGCGTGGCTAATCGTGGTAGCAGTATCAGAATACCTCGCTCTGTCGCTGAAGATAAGAAGGGTTATTTGGAAGATCGTCGACCGGCTTCAAACTGTGACCCGTACTCTGTGATCGATGCGCTCATGCGTACTTGTATTCTTAATGAATAA

Protein

MADTAHTKIEDNPKILSGPVLTNSPNAVLSKTLLSRYNDLPLPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRAIYKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQEAYDKCKDDEPWFGIEQEYILLDSDLRPFGWPPGGFPPPQGPYYCGVGANKVFARDLVEAHYRCCLYAGVPIAGTNAEVMPSQWEFQVGPSIGVHAGDDLWVARYILHRLAEEYGVIVTFDPKPVQDWNGSGAHTNFSTKKMRNDNGIIEIEKAIDKLSKVHMKHIKVYDPRGGKDNERRLTGLHETASINDFSAGVANRGSSIRIPRSVAEDKKGYLEDRRPASNCDPYSVIDALMRTCILNE

Catalytic Activity

ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine + phosphate

Subunit

Homooctamer.

Similarity

Belongs to the glutamine synthetase family.

Keywords

Alternative splicing   ATP-binding   Complete proteome   Cytoplasm   Ligase   Nucleotide-binding   Reference proteome  

Feature

chain  Glutamine synthetase 2 cytoplasmic
splice variant  In isoform A.

Uniprot

H9ITV0   I4DJ32   A0A2W1BMY2   A0A2A4J4U6   A0A0G3VIA2   S4PCI7   + More
A0A194QUW7   A0A212ETE4   A0A194PS35   H9J441   A0A2J7RJB5   D6WFH1   A0A0L7KU55   A0A068TK10   V5G3M6   A0A067R5C5   J3JTU0   A0A1Y1KMB3   A0A0M9A1M4   A0A195BGP1   A0A195C1J9   A0A151XJR8   A0A0K8VEK6   A0A034VVT9   A0A034VRN0   A0A2L1DH34   A0A0K8VUW5   E2BUV7   A0A0L7R3F9   A0A026W7I7   A0A1J1HER3   A0A2A3E9Y7   V9IDX6   A0A154P8H2   A0A088A2A8   F4WK81   A0A151JWT9   E2ATW3   A0A1I9WLM6   A0A1B6IKT6   G9C5D9   S5NFE0   A0A0M5J396   A0A0G2QRP6   A0A0A9WUK9   R4G5A5   A0A0P4VPJ5   A0A0A1XT45   A0A0A1WVL5   A0A1B6FLE3   M9V250   A0A224XIU2   W8BUA3   A0A1B6IB03   A0A1W4V0I0   U5EZ20   A0A336LVS2   A0A1W4UNL4   D0QWE3   A0A1B6EY34   A0A3B0JXI0   B4JIW8   B4L4A8   B4MCX6   E0VED6   D3TPP6   A0A0Q9WMW7   A0A0Q9VZ95   A0A1B0B749   A0A1B0AIW9   A0A1L8E2I2   A0A1A9UHR5   Q29H28   B4H0G6   C8VV58   Q16J32   Q2KQ97   B4N1T9   X2JDA5   A0A1B6CN61   B3NUD5   B4PYB1   X2JJG8   P20478   A0A1A9Y1D7   H7BUI7   A0A0L0C841   A0A3B0JXJ2   A0A1Q3G4Z2   P20478-3   Q494K4   A0A0R1EIC3   B4IK13   A0A0Q5T3A2   A0A023F924   A0A1Q3G582   A0A087UK01   A0A0V0G2W8   A0A182Q4C3   A0A069DTI5  

EC Number

6.3.1.2

Pubmed

19121390    22651552    28756777    23622113    26354079    22118469    + More
18362917    19820115    26227816    29403074    24845553    22516182    28004739    25348373    20798317    24508170    21719571    27538518    21835214    23791183    25401762    27129103    25830018    24495485    19859648    17994087    20566863    20353571    15632085    17510324    15804581    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    17550304    26109357    26109356    1969491    12537569    26108605    25474469    26334808   

EMBL

BABH01007020    AK401300    BAM17922.1    KZ150069    PZC74080.1    NWSH01003020    + More
PCG67105.1    KP657638    RSAL01000006    AKL78863.1    RVE54315.1    GAIX01004181    JAA88379.1    KQ461108    KPJ09262.1    AGBW02012593    OWR44772.1    KQ459594    KPI96122.1    BABH01032611    NEVH01002994    PNF40921.1    KQ971327    EEZ99821.1    JTDY01005646    KOB66787.1    HG965789    PYGN01000753    CDO39385.1    PSN41298.1    GALX01003841    JAB64625.1    KK852685    KDR18484.1    BT126649    AEE61612.1    GEZM01081263    GEZM01081262    JAV61728.1    KQ435762    KOX75490.1    KQ976491    KYM83318.1    KQ978379    KYM94490.1    KQ982068    KYQ60545.1    GDHF01015334    JAI36980.1    GAKP01012982    JAC45970.1    GAKP01012981    JAC45971.1    KY681699    AVD29897.1    GDHF01009632    JAI42682.1    GL450772    EFN80494.1    KQ414663    KOC65354.1    KK107374    EZA51576.1    CVRI01000001    CRK86464.1    KZ288311    PBC28530.1    JR039080    AEY58519.1    KQ434844    KZC08141.1    GL888197    EGI65384.1    KQ981622    KYN39215.1    GL442742    EFN63125.1    KU932410    APA34046.1    GECU01020177    JAS87529.1    HQ851380    AEV89758.1    KF021980    AGR65729.1    CP012528    ALC49436.1    KC344400    AGY46258.1    GBHO01033421    GBHO01005375    GBHO01005374    GBHO01005369    GBRD01014272    GBRD01011329    JAG10183.1    JAG38229.1    JAG38230.1    JAG38235.1    JAG51554.1    GAHY01000111    JAA77399.1    GDKW01001673    JAI54922.1    GBXI01000554    JAD13738.1    GBXI01011834    JAD02458.1    GECZ01018741    JAS51028.1    KC445137    AGJ70738.1    GFTR01005462    JAW10964.1    GAMC01006052    JAC00504.1    GECU01023633    JAS84073.1    GANO01001583    JAB58288.1    UFQS01000234    UFQT01000234    SSX01768.1    SSX22146.1    FJ821026    ACN94650.1    GECZ01026871    JAS42898.1    OUUW01000011    SPP86775.1    CH916370    EDV99532.1    CH933810    EDW07386.1    CH940660    EDW58048.1    DS235090    EEB11742.1    CCAG010007360    EZ423398    ADD19674.1    KRF94024.1    KRF78088.1    JXJN01009401    GFDF01001163    JAV12921.1    CH379064    EAL31931.1    CH479200    EDW29761.1    BT099804    ACV91641.1    CH478033    EAT34276.1    AY623406    AAV31915.1    EAT34277.1    CH963925    EDW78328.1    AE014298    AHN59591.1    GEDC01022402    JAS14896.1    CH954180    EDV46050.1    CM000162    EDX01973.1    AHN59592.1    X52759    AY060701    JQ679010    AFB69511.1    JRES01000777    KNC28415.1    SPP86774.1    GFDL01000209    JAV34836.1    BT023772    AAZ41780.1    KRK06487.1    CH480851    EDW51385.1    KQS29805.1    GBBI01001238    JAC17474.1    GFDL01000119    JAV34926.1    KK120178    KFM77690.1    GECL01003697    JAP02427.1    AXCN02000052    GBGD01001902    JAC86987.1   

Proteomes

UP000005204    UP000218220    UP000283053    UP000053240    UP000007151    UP000053268    + More
UP000235965    UP000007266    UP000037510    UP000245037    UP000027135    UP000053105    UP000078540    UP000078542    UP000075809    UP000008237    UP000053825    UP000053097    UP000183832    UP000242457    UP000076502    UP000005203    UP000007755    UP000078541    UP000000311    UP000092553    UP000192221    UP000268350    UP000001070    UP000009192    UP000008792    UP000009046    UP000092444    UP000092460    UP000092445    UP000078200    UP000001819    UP000008744    UP000008820    UP000007798    UP000000803    UP000008711    UP000002282    UP000092443    UP000037069    UP000001292    UP000054359    UP000075886   

PRIDE

H9ITV0    H9J441    C8VV58    X2JDA5    P20478    Q494K4    + More

Pfam

PF03951   Gln-synt_N        + More
PF00120   Gln-synt_C

Interpro

IPR008147   Gln_synt_b-grasp        + More
IPR008146   Gln_synth_cat_dom       
IPR036651   Gln_synt_N       
IPR014746   Gln_synth/guanido_kin_cat_dom       
IPR027302   Gln_synth_N_conserv_site       
IPR027303   Gln_synth_gly_rich_site       

SUPFAM

SSF55931   SSF55931        + More
SSF54368   SSF54368       

Gene 3D

3.10.20.70    

ProteinModelPortal

H9ITV0   I4DJ32   A0A2W1BMY2   A0A2A4J4U6   A0A0G3VIA2   S4PCI7   + More
A0A194QUW7   A0A212ETE4   A0A194PS35   H9J441   A0A2J7RJB5   D6WFH1   A0A0L7KU55   A0A068TK10   V5G3M6   A0A067R5C5   J3JTU0   A0A1Y1KMB3   A0A0M9A1M4   A0A195BGP1   A0A195C1J9   A0A151XJR8   A0A0K8VEK6   A0A034VVT9   A0A034VRN0   A0A2L1DH34   A0A0K8VUW5   E2BUV7   A0A0L7R3F9   A0A026W7I7   A0A1J1HER3   A0A2A3E9Y7   V9IDX6   A0A154P8H2   A0A088A2A8   F4WK81   A0A151JWT9   E2ATW3   A0A1I9WLM6   A0A1B6IKT6   G9C5D9   S5NFE0   A0A0M5J396   A0A0G2QRP6   A0A0A9WUK9   R4G5A5   A0A0P4VPJ5   A0A0A1XT45   A0A0A1WVL5   A0A1B6FLE3   M9V250   A0A224XIU2   W8BUA3   A0A1B6IB03   A0A1W4V0I0   U5EZ20   A0A336LVS2   A0A1W4UNL4   D0QWE3   A0A1B6EY34   A0A3B0JXI0   B4JIW8   B4L4A8   B4MCX6   E0VED6   D3TPP6   A0A0Q9WMW7   A0A0Q9VZ95   A0A1B0B749   A0A1B0AIW9   A0A1L8E2I2   A0A1A9UHR5   Q29H28   B4H0G6   C8VV58   Q16J32   Q2KQ97   B4N1T9   X2JDA5   A0A1B6CN61   B3NUD5   B4PYB1   X2JJG8   P20478   A0A1A9Y1D7   H7BUI7   A0A0L0C841   A0A3B0JXJ2   A0A1Q3G4Z2   P20478-3   Q494K4   A0A0R1EIC3   B4IK13   A0A0Q5T3A2   A0A023F924   A0A1Q3G582   A0A087UK01   A0A0V0G2W8   A0A182Q4C3   A0A069DTI5  

PDB

2UU7     E-value=7.07567e-131,     Score=1196

KEGG

101738265    K01915 glutamine synthetase [EC:6.3.1.2] | (RefSeq) glutamine synthetase 2 cytoplasmic-like

PATHWAY

00220    Arginine biosynthesis - Bombyx mori (domestic silkworm)
00250    Alanine, aspartate and glutamate metabolism - Bombyx mori (domestic silkworm)
00630    Glyoxylate and dicarboxylate metabolism - Bombyx mori (domestic silkworm)
00910    Nitrogen metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)
01230    Biosynthesis of amino acids - Bombyx mori (domestic silkworm)

GO

GO:0004356   GO:0005524   GO:0006542   GO:0005737   GO:0045213   GO:0007416   GO:0006538   GO:0006807   GO:0003824   GO:0007186   GO:0016021   GO:0006810   GO:0009072   GO:0007169  

Subcellular location

Cytoplasm  

Length:

383

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00489

Exp number, first 60 AAs:

0.00204

Total prob of N-in:

0.03096

outside

1  -  383

 

 

Pi

0.381603

Theta

1.893217

Tajima's D

-1.88501

CLR

0

CSRT

0.0151992400379981

Interpretation

Possibly Positive selection