Gene

KWMTBOMO00279  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA000672

Annotation

putative_citrate_synthase_[Danaus_plexippus]

Location

Bomo_Chr1(+):8878643-8893820      View in EpiBrowser

Full name

Citrate synthase       + More
Probable citrate synthase 2, mitochondrial      
Probable citrate synthase 1, mitochondrial      
Probable citrate synthase, mitochondrial      

Alternative Name

Protein knockdown

Location in the cell

Mitochondrial   Reliability : 3.614

CDS

ATGGCTCTATTCAGGATCACATCTTCAAGACTCGTAGAATTGCAGAAAGCATGTCCGACTGCGACAGTTCTACTTCGAGGTCTAAGCGCTGAACAGACGAATTTAAAAAGTATTCTCCAGGAAAAGATTCCCAAGGAACAAGAGAAGATCCGCGAATTCCGAAAGAAGCATGGTTCCACTAAAGTCGGAGAGGTCACAGTTGATATGATGTACGGTGGCATGCGTGGAATCAAAGGTCTGGTTTGGGAAACCTCTGTGCTGGATGCCGATGAAGGAATCCGTTTCCGTGGTCTATCCATCCCTGAGTGCCAACAGCAACTGCCCAAGGCTAAGGGTGGAGAAGAACCACTACCCGAAGGTCTTTTCTGGCTTCTCGTCACTGGCGATATACCCACCGAAGCGCAAGCTAAAGCGCTGTCTAAAGAATGGGCAGCGAGGGCGGAGCTACCGGCTCACGTAGTGACAATGTTGAACAATATGCCCGGCAAACTGCATCCCATGTCACAGTTCTCGGCTGCCGTCACCGCACTCAACAGTGAATCTAAATTCGCTAAAGCCTACTCAGAGGGCGTGCACAAATCCAAGTATTGGGAGTACGTGTACGAAGACACAATGAACCTTATCGCGAAACTGCCCGTGATCGCCGCCACTATCTATCGCAACGTGTACCGCGATGGCAAGGGCATCGGCGCCATCGACGACAACAAGGACTGGTCCGCGAACTACTGCAACATGCTCGGCTTCGACGACCCGCAGTTCACCGAACTCATGCGTCTCTACCTCACCATACACAGTGACCACGAGGGTGGCAACGTTTCTGCGCACACTACGCATTTAGTGGGTTCTGCTCTTAGTGATCCTTACTTATCTTTCGCCGCTGGACTCAATGGTCTTGCTGGACCACTTCACGGCCTGGCTAATCAAGAGGTTCTGGTATGGCTCGAGAAACTGCGCAAACAAGTTGGTGATAATTTCACAGAAGAGCAACTCAAAGAATTCATCTGGAAAACACTTAAATCTGGTCAGGTTGTACCTGGTTACGGTCACGCAGTACTTAGAAAAACTGATCCAAGATATACTTGCCAGCGTGAGTTTGCTCTTAAGCACTTACCCAATGACCCATTATTCAAGCTGGTCGCTGCTGTTTACAAGGTTGTTCCGCCGATCCTCACCGAACTTGGCAAAGTCAAGAACCCATGGCCTAATGTAGACTCCCATTCGGGAGTTCTTTTGCAGTATTATGGTCTGAAGGAGATGAACTACTATACAGTAATGTTTGGAGTGTCCCGAGCGTTGGGTGTTCTCGCTCAGTTGATTTGGTCCCGTGCGCTCGGACTTCCCATTGAGAGACCAAAGTCGCTCAGCACGGAGATGCTTATGAAACAAGTCGGGAAGTAA

Protein

MALFRITSSRLVELQKACPTATVLLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHGSTKVGEVTVDMMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTGDIPTEAQAKALSKEWAARAELPAHVVTMLNNMPGKLHPMSQFSAAVTALNSESKFAKAYSEGVHKSKYWEYVYEDTMNLIAKLPVIAATIYRNVYRDGKGIGAIDDNKDWSANYCNMLGFDDPQFTELMRLYLTIHSDHEGGNVSAHTTHLVGSALSDPYLSFAAGLNGLAGPLHGLANQEVLVWLEKLRKQVGDNFTEEQLKEFIWKTLKSGQVVPGYGHAVLRKTDPRYTCQREFALKHLPNDPLFKLVAAVYKVVPPILTELGKVKNPWPNVDSHSGVLLQYYGLKEMNYYTVMFGVSRALGVLAQLIWSRALGLPIERPKSLSTEMLMKQVGK

Description

Plays a role in controlling neuronal activity and seizure susceptibility.

Catalytic Activity

acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+)

Subunit

Homodimer.

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.
Mutation of kdn causes a behavioral mutation ('bang sensitivity' = temporarily paralysis in response to a physical jolt). Flies lacking kdn exhibit alterations in neuronal firing patterns in the giant fiber (GF) pathway.

Similarity

Belongs to the citrate synthase family.

Keywords

Complete proteome   Mitochondrion   Reference proteome   Transferase   Transit peptide   Tricarboxylic acid cycle   Alternative splicing   Behavior  

Feature

chain  Probable citrate synthase 2, mitochondrial
splice variant  In isoform B.

Uniprot

H9ITU2   A0A2A4JPM9   A0A212ETD4   A0A2A4JNS8   A0A2H1VNY9   A0A194QWB0   + More
A0A194PS44   J3JV43   U4UCM5   A0A1Y1K9C2   A0A1W4WQY7   D6WMM4   A0A023ESP1   N6U883   A0A2J7RJY2   A0A084WJ30   A0A2Z5RE60   Q16P20   U5EUY0   A0A182PV63   Q17GM7   V5G175   A0A1I8JTB6   A0A182UVT4   A0A182KTE7   Q7PV48   A0A182RSS8   A0A336M4V0   A0A182N3L6   A0A182Q2D7   A0A1Y9IVZ9   A0A1Y9J0F6   T1E9V8   A0A2M3Z0V5   T1E2L3   A0A2M4A7U9   A0A2M4A7S6   A0A2M4CUN3   A0A1Q3FUN5   A0A1W5YLL6   A0A182K1V2   A0A182FVT2   A0A182GKM3   A0A182YCD2   A0A1J1IJG4   A0A3Q0IKK7   A0A182M257   A0A1Q3FV06   A0A182JN40   W5J9I7   A0A1W6EVT2   A0A3L8D9H6   A0A1B6LTM4   A0A1B6ERI7   A0A1S3CUV0   A0A1B6FW63   A0A0K8TSU1   A0A1L8E0W5   A0A1L8E196   A0A158NK90   A0A1L8E0T5   A0A1L8EE92   A0A067RFE0   A0A1L8EEN0   T1PDY8   B4N2K9   A0A069DTW1   A0A0P4VSB3   R4G5K7   A0A1B0D970   A0A0V0G5W9   A0A1B6CDS0   A0A224XBZ6   A0A0A1XDV0   A0A0J7NLK9   V5SJ12   A0A195FBS5   D3TQS9   A0A0P4WHU5   Q0QHL3   A0A1I8PUP1   W8CC68   A0A0A9VZC2   A0A023F8N9   R4WJI6   E2BRV0   A0A026X2M5   A0A0P8XS56   A0A151X6U5   A0A1W4VF19   A0A1I9WLM9   E2AVF4   C6TP50   B3NXS1   A0A0M5JAP7   A0A1B6JC96   A0A0R3NYW2   E0VRF1   Q9W401   B4L2G7  

EC Number

2.3.3.16

Pubmed

19121390    22118469    26354079    22516182    23537049    28004739    + More
18362917    19820115    24945155    24438588    26760975    17510324    20966253    12364791    14747013    17210077    24330624    26483478    25244985    20920257    23761445    30249741    26369729    21347285    24845553    25315136    17994087    18057021    26334808    27129103    25830018    20353571    16907828    24495485    25401762    26823975    25474469    23691247    20798317    24508170    27538518    15632085    23185243    20566863    10731132    12537572    12537569    14680800    16648587   

EMBL

BABH01006978    NWSH01000884    PCG73716.1    AGBW02012593    OWR44762.1    PCG73715.1    + More
ODYU01003579    SOQ42518.1    KQ461108    KPJ09250.1    KQ459594    KPI96132.1    BT127109    AEE62071.1    KB632297    ERL91704.1    GEZM01088604    JAV58072.1    KQ971343    EFA03279.1    GAPW01001305    JAC12293.1    APGK01044816    APGK01044817    APGK01044818    KB741028    ENN74802.1    NEVH01002982    PNF41146.1    ATLV01023968    KE525347    KFB50224.1    FX983734    BAX07233.1    CH477799    GANO01001257    JAB58614.1    CH477258    GALX01004701    JAB63765.1    APCN01002450    AAAB01008986    EAA00454.5    UFQS01000559    UFQT01000559    SSX04940.1    SSX25302.1    AXCN02001106    GAMD01001563    JAB00028.1    GGFM01001411    MBW22162.1    GALA01001160    JAA93692.1    GGFK01003499    MBW36820.1    GGFK01003508    MBW36829.1    GGFL01004370    MBW68548.1    GFDL01003726    JAV31319.1    KX147674    ARI45078.1    JXUM01070227    JXUM01070228    JXUM01070229    JXUM01070230    JXUM01070231    JXUM01070232    KQ562595    KXJ75524.1    CVRI01000054    CRL00383.1    AXCM01005459    GFDL01003729    JAV31316.1    ADMH02001988    ETN60073.1    KY563412    ARK19821.1    QOIP01000011    RLU16539.1    GEBQ01012937    JAT27040.1    GECZ01029227    JAS40542.1    GECZ01015486    JAS54283.1    GDAI01000164    JAI17439.1    GFDF01001743    JAV12341.1    GFDF01001742    JAV12342.1    ADTU01018740    ADTU01018741    GFDF01001744    JAV12340.1    GFDG01001880    JAV16919.1    KK852498    KDR22581.1    GFDG01001695    JAV17104.1    KA646158    AFP60787.1    CH963925    EDW78598.1    KRF98915.1    GBGD01001356    JAC87533.1    GDKW01003700    JAI52895.1    ACPB03000853    GAHY01000455    JAA77055.1    AJVK01004260    AJVK01004261    GECL01002680    JAP03444.1    GEDC01025705    JAS11593.1    GFTR01006501    JAW09925.1    GBXI01012403    GBXI01004713    JAD01889.1    JAD09579.1    LBMM01003579    KMQ93360.1    KF647629    AHB50491.1    KQ981693    KYN37662.1    EZ423781    ADD20057.1    GDRN01071936    JAI63621.1    DQ374006    GAMC01004166    GAMC01004165    JAC02390.1    GBHO01045634    GBRD01011409    GDHC01020673    JAF97969.1    JAG54415.1    JAP97955.1    GBBI01000972    JAC17740.1    AK417710    BAN20925.1    GL450042    EFN81583.1    KK107021    EZA62343.1    CH902630    KPU77480.1    KQ982472    KYQ56115.1    KU932413    APA34049.1    GL443090    EFN62588.1    BT099536    ACU32620.1    CH954180    EDV47372.1    CP012528    ALC49224.1    GECU01010846    JAS96860.1    CH379063    KRT06226.1    DS235465    EEB15957.1    AE014298    AY089318    CH933810    EDW06843.1   

Proteomes

UP000005204    UP000218220    UP000007151    UP000053240    UP000053268    UP000030742    + More
UP000192223    UP000007266    UP000019118    UP000235965    UP000030765    UP000008820    UP000075885    UP000075840    UP000075903    UP000075882    UP000007062    UP000075900    UP000075884    UP000075886    UP000075920    UP000076407    UP000075881    UP000069272    UP000069940    UP000249989    UP000076408    UP000183832    UP000079169    UP000075883    UP000075880    UP000000673    UP000279307    UP000005205    UP000027135    UP000095301    UP000007798    UP000015103    UP000092462    UP000036403    UP000078541    UP000092444    UP000095300    UP000008237    UP000053097    UP000007801    UP000075809    UP000192221    UP000000311    UP000008711    UP000092553    UP000001819    UP000009046    UP000000803    UP000009192   

PRIDE

H9ITU2    Q16P20    Q17GM7    Q0QHL3    C6TP50    Q9W401    + More

Pfam

PF00285   Citrate_synt        + More
PF00535   Glycos_transf_2
PF02213   GYF

Interpro

IPR019810   Citrate_synthase_AS        + More
IPR016143   Citrate_synth-like_sm_a-sub       
IPR036969   Citrate_synthase_sf       
IPR010109   Citrate_synthase_euk       
IPR002020   Citrate_synthase       
IPR016142   Citrate_synth-like_lrg_a-sub       
IPR011989   ARM-like       
IPR016024   ARM-type_fold       
IPR035518   DPG_synthase       
IPR001173   Glyco_trans_2-like       
IPR029044   Nucleotide-diphossugar_trans       
IPR003169   GYF       
IPR035445   GYF-like_dom_sf       

SUPFAM

SSF48256   SSF48256        + More
SSF48371   SSF48371       
SSF53448   SSF53448       
SSF55277   SSF55277       

Gene 3D

1.10.230.10     1.10.580.10     1.25.10.10     3.90.550.10     3.30.1490.40    

CDD

cd04188  DPG_synthase

ProteinModelPortal

H9ITU2   A0A2A4JPM9   A0A212ETD4   A0A2A4JNS8   A0A2H1VNY9   A0A194QWB0   + More
A0A194PS44   J3JV43   U4UCM5   A0A1Y1K9C2   A0A1W4WQY7   D6WMM4   A0A023ESP1   N6U883   A0A2J7RJY2   A0A084WJ30   A0A2Z5RE60   Q16P20   U5EUY0   A0A182PV63   Q17GM7   V5G175   A0A1I8JTB6   A0A182UVT4   A0A182KTE7   Q7PV48   A0A182RSS8   A0A336M4V0   A0A182N3L6   A0A182Q2D7   A0A1Y9IVZ9   A0A1Y9J0F6   T1E9V8   A0A2M3Z0V5   T1E2L3   A0A2M4A7U9   A0A2M4A7S6   A0A2M4CUN3   A0A1Q3FUN5   A0A1W5YLL6   A0A182K1V2   A0A182FVT2   A0A182GKM3   A0A182YCD2   A0A1J1IJG4   A0A3Q0IKK7   A0A182M257   A0A1Q3FV06   A0A182JN40   W5J9I7   A0A1W6EVT2   A0A3L8D9H6   A0A1B6LTM4   A0A1B6ERI7   A0A1S3CUV0   A0A1B6FW63   A0A0K8TSU1   A0A1L8E0W5   A0A1L8E196   A0A158NK90   A0A1L8E0T5   A0A1L8EE92   A0A067RFE0   A0A1L8EEN0   T1PDY8   B4N2K9   A0A069DTW1   A0A0P4VSB3   R4G5K7   A0A1B0D970   A0A0V0G5W9   A0A1B6CDS0   A0A224XBZ6   A0A0A1XDV0   A0A0J7NLK9   V5SJ12   A0A195FBS5   D3TQS9   A0A0P4WHU5   Q0QHL3   A0A1I8PUP1   W8CC68   A0A0A9VZC2   A0A023F8N9   R4WJI6   E2BRV0   A0A026X2M5   A0A0P8XS56   A0A151X6U5   A0A1W4VF19   A0A1I9WLM9   E2AVF4   C6TP50   B3NXS1   A0A0M5JAP7   A0A1B6JC96   A0A0R3NYW2   E0VRF1   Q9W401   B4L2G7  

PDB

5UQS     E-value=0,     Score=1868

KEGG

101737646    K01647 citrate synthase [EC:2.3.3.1] | (RefSeq) probable citrate synthase 2, mitochondrial

PATHWAY

00020    Citrate cycle (TCA cycle) - Bombyx mori (domestic silkworm)
00630    Glyoxylate and dicarboxylate metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)
01200    Carbon metabolism - Bombyx mori (domestic silkworm)
01210    2-Oxocarboxylic acid metabolism - Bombyx mori (domestic silkworm)
01230    Biosynthesis of amino acids - Bombyx mori (domestic silkworm)

GO

GO:0006099   GO:0004108   GO:0006101   GO:0016021   GO:0005975   GO:0005759   GO:0016740   GO:0007610   GO:0005739   GO:0046912   GO:0005506   GO:0009072   GO:0006810   GO:0007169  

PANTHER

PTHR11739  

Subcellular location

Mitochondrion matrix  

Length:

465

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

1.46415

Exp number, first 60 AAs:

0.00022

Total prob of N-in:

0.01455

outside

1  -  465

 

 

Pi

19.90474

Theta

16.527872

Tajima's D

0.181385

CLR

0

CSRT

0.421228938553072

Interpretation

Uncertain