Gene

KWMTBOMO00186

Pre Gene Modal

BGIBMGA000620

Annotation

heparanase-like_protein_[Bombyx_mori]

Location

Bomo_Chr1(+):5963213-5965045      View in EpiBrowser

Full name

Heparanase      

Location in the cell

PlasmaMembrane   Reliability : 2.273

CDS

ATGATGTCGAATAAACGATTAGTAGCTACATGTGCGATAGCATTTTGCTGCAACGTGGCTATGCTCGTATTATTTGTAAAATACAACGGCGGTGTAAGGTACTTTGTTACGATTAATGAAAATCAAGAAGATATCAAGCTAATCTCGGAAGACTTTCTCAGCTTTGGGATTGACACCATTGAAATTGAGAACTACAACAGAATTAACTATTCGGACACTAGGTTGAGGGAGTTAGCAGCAGCACTATCCCCAGCGCGGCTTCGTCTTGGCGGCACCATGTCTGAACGTCTCATATTTAGTAAAGAAAATATCCCAATTTCATGCCACAATTGTAGTTATAAATCTTATCCAAAATCTTTATGCCAACTTATTGAAAAACCATGTAAACACAAACACAAGTTTTTACCCTTCTTTATAATGACTGGAAATGAATGGAACCAAATTAACGATTTTTGCAGAAAGACTAATCTAAAATTGTTATTCTCGTTGAATGCAATGCTTCGTGATAATCATGGTTGGAATGAAAAGAATGCTAGAGAACTTATTGAATTTTCAAAACACAAACAGTATGCTATTGATTGGCAGTTAGGAAACGAGCCAAACTCTTTTCAACATGTTTTTAATGAAAGTGTAACTCCTCAAATATTAGCCAAGGACTTTGAAAAGCTACGTAAACTTTTGAATCATAATGGTTACAGACATTCTCTGATTGTCGGCCCAGACACAACAAGACCACAACCACACCGTCCTGAATGCCTTAAATATATGATAGAATTTTTAGGTAATGGATCTCATTATATAAATGTAAGATCATGGCATCAGTATTATTTAAACAGTAAAACTGCCAAATTGGAGGATTTTTGGAATCCAGAAACATTTGATTTGCTCAGACAGCAAATAGAAACAATGCAAAATCAAACCAAGAAGTACAAAAATATCCCAATGTGGCTAAGTGAAACTAGTAGCTCATATGGAGGTGGTGCACCAGGATTATCAAATACTTATGCAGGTAGCCCGCTTTGGATTGATAAACTAGGTCTATCGGCAAAATACAATATATCCACCGTCATTCGACAAAGCTTTATTGGTGGATATTACAGCCTAGTGGATGAAAATCTGAAACCCTTACCAGATTGGTGGATCAGTGTCTTGTATAAAAAATTGGTTGGAAATAAAGTTTTACAGGTGCAATGCAATTGCTCAAGGTTTCAAAGACTGTATATTCATTGCACCAACAGAAAATACACTAATGACACATCAGCTGTAACACTGTATGGTGTTAATTTGGAAATGGCAAAAGCTCGATTCTTCCTTAATGGCACTGCTTTACATGGAGACGATTTAATAATTCATGAATATATCATAAGTGCACCTTCAAATAACCGAAAATCAAAAACAATATTGTTAAATGGCTGGCCACTTTATTATGAATCTAATTTACACAATTTGCGGCCTAATATTCATAGGTATGGACGCTATGTTTCACTACCTCCATATTCTATTGGATTTTGGGTAATTAAGAAAACATCAATTACTGTATGCGAATAA

Protein

MMSNKRLVATCAIAFCCNVAMLVLFVKYNGGVRYFVTINENQEDIKLISEDFLSFGIDTIEIENYNRINYSDTRLRELAAALSPARLRLGGTMSERLIFSKENIPISCHNCSYKSYPKSLCQLIEKPCKHKHKFLPFFIMTGNEWNQINDFCRKTNLKLLFSLNAMLRDNHGWNEKNARELIEFSKHKQYAIDWQLGNEPNSFQHVFNESVTPQILAKDFEKLRKLLNHNGYRHSLIVGPDTTRPQPHRPECLKYMIEFLGNGSHYINVRSWHQYYLNSKTAKLEDFWNPETFDLLRQQIETMQNQTKKYKNIPMWLSETSSSYGGGAPGLSNTYAGSPLWIDKLGLSAKYNISTVIRQSFIGGYYSLVDENLKPLPDWWISVLYKKLVGNKVLQVQCNCSRFQRLYIHCTNRKYTNDTSAVTLYGVNLEMAKARFFLNGTALHGDDLIIHEYIISAPSNNRKSKTILLNGWPLYYESNLHNLRPNIHRYGRYVSLPPYSIGFWVIKKTSITVCE

Description

Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. Essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans. Acts as procoagulant by enhancing the generation of activated factor X/F10 in the presence of tissue factor/TF and activated factor VII/F7. Independent of its enzymatic activity, increases cell adhesion to the extracellular matrix (ECM). Enhances AKT1/PKB phosphorylation, possibly via interaction with a lipid raft-resident receptor. Plays a role in the regulation of osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis (By similarity).

Catalytic Activity

Endohydrolysis of (1->4)-beta-D-glycosidic bonds of heparan sulfate chains in heparan sulfate proteoglycan.

Subunit

Heterodimer; heterodimer formation between the 8 kDa and the 50 kDa subunits is required for enzyme activity (By similarity). Interacts with TF; the interaction, inhibited by heparin, enhances the generation of activated factor X and activates coagulation. Interacts with HRG; the interaction is enhanced at acidic pH, partially inhibits binding of HPSE to cell surface receptors and modulates its enzymatic activity. Interacts with SDC1; the interaction enhances the shedding of SDC1. Interacts with HPSE2 (By similarity).

Similarity

Belongs to the glycosyl hydrolase 79 family.

Keywords

Calcium   Cell adhesion   Complete proteome   Disulfide bond   Glycoprotein   Hydrolase   Lysosome   Magnesium   Membrane   Nucleus   Reference proteome   Secreted   Signal  

Feature

chain  Heparanase 8 kDa subunit
propeptide  Linker peptide

Uniprot

Q8T108   H9ITP0   A0A2A4JSC4   A0A2H1VBP0   A0A0L7LS66   A0A194QV50   + More
A0A194PTW0   A0A212EMQ1   A0A2J7R2X5   A0A1Y1N3N1   A0A067RI03   A0A139WIE2   K7J4B8   A0A151WKK8   A0A0C9RBP0   A0A232F8N2   A0A158NZM7   E2A4R0   F4W9X3   A0A088AF83   A0A3L8E003   A0A026WAL6   A0A195BQ80   A0A195EDM9   A0A2A3EP98   A0A195EZA9   A0A0L7QYL3   A0A154P219   A0A151IKA7   A0A0J7KBC1   E9IFP7   E2BB54   A0A1Y1KAI0   A0A1S3HCB2   N6T7N5   W5N2L0   A0A1S3L1K1   A0A2U3YEC9   A0A1Y1K9K5   A0A3B3ZGD4   F1RVE1   A0A2Y9I6Y0   A0A2D0T167   A0A340WKN8   A0A2U3VBE0   A0A337SQG9   A0A2Y9S152   A0A091UNA3   A0A2U3V8L3   H3CJF0   A0A2D0T089   A0A341CA55   A0A3B4BWW5   A0A2Y9QIL2   A0A091TVT7   A0A091H689   A0A093CV92   H2VEW5   A0A1S3LT31   A0A0F8AB04   N6TKX2   C0LJM7   W5UG18   A0A1S3LRZ8   A0A087R3Y4   H0YVD2   A0A099Z0F6   A0A087VB61   B1H188   A0A383YYQ7   A0A3L8SFS9   A0A091IDF6   A0A093PB95   M3XIZ7   A0A3P9DAR6   G3SLF1   A0A091S335   A0A3P8ZD73   H3APX2   A0A3Q0JNT6   Q9MYY0   A0A091UG24   A0A3B1KAW3   R7VP50   N6SZ85   A0A3Q0CCN7   A0A2I0MGN3   A0A091PR16   A0A3B3QFY4   A0A3B4FTE3   T1HEG7   A0A1W5A8L1   G1KKK3   A0A3P8U1I5   A0A218UTW9   F7BSR7   F7BST4   A0A1A7YIA2   A0A0M9AA15  

EC Number

3.2.1.166

Pubmed

12715162    19121390    26227816    26354079    22118469    28004739    + More
24845553    18362917    19820115    20075255    28648823    21347285    20798317    21719571    30249741    24508170    21282665    23537049    25463417    17975172    15496914    21551351    25835551    19567219    23127152    20360741    30282656    9215903    25186727    25069045    11277877    25329095    23371554    28071753    29240929    20431018   

EMBL

AB079860    AB090307    BAB85191.1    BABH01006749    NWSH01000699    PCG74729.1    + More
ODYU01001673    SOQ38221.1    JTDY01000220    KOB78224.1    KQ461108    KPJ09194.1    KQ459594    KPI96194.1    AGBW02013811    OWR42746.1    NEVH01007825    PNF35173.1    GEZM01015666    JAV91470.1    KK852669    KDR18877.1    KQ971338    KYB27768.1    AAZX01006389    KQ983012    KYQ48376.1    GBYB01013954    JAG83721.1    NNAY01000718    OXU26819.1    ADTU01000889    GL436716    EFN71594.1    GL888033    EGI69109.1    QOIP01000002    RLU25388.1    KK107321    EZA52706.1    KQ976423    KYM88748.1    KQ979039    KYN23330.1    KZ288199    PBC33633.1    KQ981909    KYN33209.1    KQ414688    KOC63705.1    KQ434803    KZC05907.1    KQ977234    KYN04707.1    LBMM01010275    KMQ87559.1    GL762889    EFZ20607.1    GL446916    EFN87075.1    GEZM01090927    JAV57160.1    APGK01049250    KB741146    KB631697    ENN73698.1    ERL85442.1    AHAT01017247    GEZM01090926    JAV57161.1    AEMK02000067    AANG04001605    KL409714    KFQ91370.1    KK466795    KFQ82336.1    KL526587    KFO91366.1    KL469781    KFV18413.1    KQ046811    KKF09058.1    APGK01033057    KB740860    KB632287    ENN78583.1    ERL91469.1    FJ713408    ACN62224.1    JT414150    AHH40514.1    KL226085    KFM08188.1    ABQF01048910    KL886919    KGL74255.1    KL486771    KFO09853.1    BC160516    AAI60516.1    QUSF01000025    RLW00832.1    KL218525    KFP06282.1    KL225236    KFW69532.1    AFYH01102064    AFYH01102065    AFYH01102066    KK940905    KFQ50576.1    AF281160    KK433798    KFQ89679.1    KB375900    EMC79969.1    APGK01058678    KB741291    KB631579    ENN70553.1    ERL84425.1    AKCR02000013    PKK28843.1    KK664513    KFQ10352.1    ACPB03022058    MUZQ01000144    OWK56890.1    AAMC01058517    AAMC01058518    HADW01018856    HADX01007724    SBP29956.1    KQ435706    KOX80277.1   

Proteomes

UP000005204    UP000218220    UP000037510    UP000053240    UP000053268    UP000007151    + More
UP000235965    UP000027135    UP000007266    UP000002358    UP000075809    UP000215335    UP000005205    UP000000311    UP000007755    UP000005203    UP000279307    UP000053097    UP000078540    UP000078492    UP000242457    UP000078541    UP000053825    UP000076502    UP000078542    UP000036403    UP000008237    UP000085678    UP000019118    UP000030742    UP000018468    UP000087266    UP000245341    UP000261520    UP000008227    UP000248481    UP000221080    UP000265300    UP000245340    UP000011712    UP000248484    UP000053283    UP000245320    UP000007303    UP000252040    UP000261440    UP000248483    UP000005226    UP000053286    UP000007754    UP000053641    UP000261681    UP000276834    UP000054308    UP000054081    UP000008672    UP000265160    UP000007646    UP000265140    UP000009136    UP000018467    UP000189706    UP000053872    UP000261540    UP000261460    UP000015103    UP000192224    UP000001646    UP000265080    UP000197619    UP000008143    UP000053105   

PRIDE

Q8T108    H9ITP0    F1RVE1    Q9MYY0   

Pfam

PF03662   Glyco_hydro_79n

Interpro

IPR017853   Glycoside_hydrolase_SF        + More
IPR005199   Glyco_hydro_79       

SUPFAM

SSF51445   SSF51445       

ProteinModelPortal

Q8T108   H9ITP0   A0A2A4JSC4   A0A2H1VBP0   A0A0L7LS66   A0A194QV50   + More
A0A194PTW0   A0A212EMQ1   A0A2J7R2X5   A0A1Y1N3N1   A0A067RI03   A0A139WIE2   K7J4B8   A0A151WKK8   A0A0C9RBP0   A0A232F8N2   A0A158NZM7   E2A4R0   F4W9X3   A0A088AF83   A0A3L8E003   A0A026WAL6   A0A195BQ80   A0A195EDM9   A0A2A3EP98   A0A195EZA9   A0A0L7QYL3   A0A154P219   A0A151IKA7   A0A0J7KBC1   E9IFP7   E2BB54   A0A1Y1KAI0   A0A1S3HCB2   N6T7N5   W5N2L0   A0A1S3L1K1   A0A2U3YEC9   A0A1Y1K9K5   A0A3B3ZGD4   F1RVE1   A0A2Y9I6Y0   A0A2D0T167   A0A340WKN8   A0A2U3VBE0   A0A337SQG9   A0A2Y9S152   A0A091UNA3   A0A2U3V8L3   H3CJF0   A0A2D0T089   A0A341CA55   A0A3B4BWW5   A0A2Y9QIL2   A0A091TVT7   A0A091H689   A0A093CV92   H2VEW5   A0A1S3LT31   A0A0F8AB04   N6TKX2   C0LJM7   W5UG18   A0A1S3LRZ8   A0A087R3Y4   H0YVD2   A0A099Z0F6   A0A087VB61   B1H188   A0A383YYQ7   A0A3L8SFS9   A0A091IDF6   A0A093PB95   M3XIZ7   A0A3P9DAR6   G3SLF1   A0A091S335   A0A3P8ZD73   H3APX2   A0A3Q0JNT6   Q9MYY0   A0A091UG24   A0A3B1KAW3   R7VP50   N6SZ85   A0A3Q0CCN7   A0A2I0MGN3   A0A091PR16   A0A3B3QFY4   A0A3B4FTE3   T1HEG7   A0A1W5A8L1   G1KKK3   A0A3P8U1I5   A0A218UTW9   F7BSR7   F7BST4   A0A1A7YIA2   A0A0M9AA15  

PDB

5LA7     E-value=5.07643e-62,     Score=604

KEGG

100141461    K07964 heparanase [EC:3.2.1.166] | (RefSeq) Hepa, Bmhepa; heparanase-like protein

PATHWAY

00531    Glycosaminoglycan degradation - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0016020   GO:0016798   GO:0016021   GO:0031012   GO:0061042   GO:0030194   GO:0051798   GO:0007160   GO:0005654   GO:0060055   GO:0010575   GO:0030200   GO:0045121   GO:0046983   GO:0030305   GO:0033690   GO:0005764   GO:0045545   GO:0051897   GO:0005634   GO:0005765   GO:0005975   GO:0016192   GO:0031251   GO:0004672   GO:0003707   GO:0007169  

Subcellular location

Lysosome membrane   Proheparanase is secreted via vesicles of the Golgi. Interacts with cell membrane heparan sulfate proteoglycans (HSPGs). Endocytosed and accumulates in endosomes. Transferred to lysosomes where it is proteolytically cleaved to produce the active enzyme. Under certain stimuli, transferred to the cell surface. Associates with lipid rafts. Colocalizes with SDC1 in endosomal/lysosomal vesicles. Accumulates in perinuclear lysosomal vesicles. Heparin retains proheparanase in the extracellular medium (By similarity).   With evidence from 1 publications.
Secreted   Proheparanase is secreted via vesicles of the Golgi. Interacts with cell membrane heparan sulfate proteoglycans (HSPGs). Endocytosed and accumulates in endosomes. Transferred to lysosomes where it is proteolytically cleaved to produce the active enzyme. Under certain stimuli, transferred to the cell surface. Associates with lipid rafts. Colocalizes with SDC1 in endosomal/lysosomal vesicles. Accumulates in perinuclear lysosomal vesicles. Heparin retains proheparanase in the extracellular medium (By similarity).   With evidence from 1 publications.
Nucleus   Proheparanase is secreted via vesicles of the Golgi. Interacts with cell membrane heparan sulfate proteoglycans (HSPGs). Endocytosed and accumulates in endosomes. Transferred to lysosomes where it is proteolytically cleaved to produce the active enzyme. Under certain stimuli, transferred to the cell surface. Associates with lipid rafts. Colocalizes with SDC1 in endosomal/lysosomal vesicles. Accumulates in perinuclear lysosomal vesicles. Heparin retains proheparanase in the extracellular medium (By similarity).   With evidence from 1 publications.

Length:

515

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

20.09637

Exp number, first 60 AAs:

20.07637

Total prob of N-in:

0.99543

POSSIBLE N-term signal

sequence

inside

1  -  6

TMhelix

7  -  26

outside

27  -  515

 

 

Pi

7.530337

Theta

7.320019

Tajima's D

0.309261

CLR

0

CSRT

0.471676416179191

Interpretation

Uncertain