Gene

KWMTBOMO00156

Pre Gene Modal

BGIBMGA001996

Annotation

PREDICTED:_nervous_wreck_isoform_X1_[Bombyx_mori]

Location

Bomo_Chr1(-):4831835-4927123      View in EpiBrowser

Full name

Protein nervous wreck      

Location in the cell

Nuclear   Reliability : 2.301

CDS

ATGTGGAATGTGTGGCGCACGGTGCTGGAAGAGAACGAGAAGCTAGCACGCGCTCGTCTCGCAGCCGTCGAAGTGTTCCAACAGCACATCACCGATGAAGCGAAGCTGCTCCGACACCAGAAGCTCAGCTCTGCTAGGAAGTTTACGGAGGGCCTTGCTCAAGCCCACAAGGAGCTGCAGATGACTGTAGGTGAAGTTGACAAGACCAAGAAGCTGTACTTCGACGAGGAGCATACTGCTCATGACGTGCGCGACAAAGCTAAAGACATCGAGGAGAATAAACCGAGTAGCTCCCGGGGATCACCGAAACATCAACCGCCAGAAAGGAATGAACTAGTTGTAGATAAATATTGGTTGAAGAAGAAAAAAGGATCGTTCTTCCAATCGATTACTTCGCTGCAGAAGAACAGCGCCAAGGTTTCGTCCCGTCGTGATCAGCTGGAGGAGAAATCCACTGGCGCCAGGAACGATTATATTCTAAGCATTGCAGCTGCTAATGCTCACCAGAATCGCTACTTCCTAGTGGATCTTCAGACTTGCATGCAAAACATGGAGTCCTCGGTGTACGAGAAGGTTTCTGAGTACCTTACGCTTATGGGACGCACGGAACTCTTGACTTGCTCTGCCACCCAGCACTCATTTGGGAAGATCAGGGATCAAGCGCAGCAGCTGACCAGGGAATATAACTTGCAATGCTTGTATTTATATTATCCAGTGCTGAAGCAACATATCCAGTACGAGTTCGAGCCTTGCGACAATGATCCGATTGACACTATCACCATGGAGCACGAATCCGTGGCTGTCACTTTGGCTCAAGAGGCTCGCCGCTGGGCCACCCGCGTAGTTCGCGAGGCATCCCTCGTCCGGGATGCGAATCGCAAAATCAGTTCTTATCAAGCTATGCGGGAGTCTGGACATAAGGTGGACCCGAACGAACCCAACGGACCTGAGTTGGAAGTAAAGATGGATGAATTGCGTGCCACAATCCGTCGCTCTGAAATATCTAAGGCCAAGTACGAAGCTCGACTGGAATGCTTAAGAGTCGGAGGTGCTCCTGTAGATGATTGGCTAAAGGAAATCGATCTGCTGGCTGTCCAGGAGACACTGCCACGCAGCAGCAGCCTGCTAAGTGTTAGAACTGACGCATCCGGAGCCGCCGACCAGCCGAGCTCGGACTCTTTCTATGATAGCGACAACACTGAGGGTGAAGCAGCTGCTTCGGTGGCCGGCACCAGTGGCTCTGGTGGCCACCAACGTACCACTTCGGGCTCTCAGCATGAGGACGATCATGAAGATGAGGTTGACGCTGCCCTTGAGATTGAACGCCAGCGAATCGAGCAACTTGCGGGAGGATGGGATGATCCCACTCAAGTGAACTGGGGTGAATCCGACCCAGAGCCCTCTACTTCAGAGCCTATAGAACCACCAGCACCACCGCTTTACAAGTGTACTGCTCTTTACTCTTACACCGCTCAGAATCCGGATGAGTTGTCTATCATTGAGAACGAACAGCTTGAAGTGGTTGGAGAAGGCGATGGTGACGGTTGGCTGAAAGCTCGGAACTATCGCGGAGAAGAAGGCTACGTGCCACACAACTATCTCGATGTTGACCATGATCAGGCATCTAGCGCTCCAGGTTTGGTAAGCCAAATATCTTTCTCTTCTGTGGATTACACTGTGGAGGGTGAAGACGCTGACGTTGTCCAGTCACCGGATCAGATCTCTGTGATTTCGGCCCCCGTCGGCAAGCCTGATGAACCGACTAAAGCCGCAGAAGCAGCTCAGGGCGAGGCCGCTGCTGTAGCCCAGGGGCCACCAAAAGTCGACCTACCGACTCTCGGTTACTGCTTTGCTCTTTACGATTACGAAGCTGAGGCGTGCGATGAGCTCAACCTTGAAGAGGGACAGATTATTCGCGTTGTGTCCCGTAATGCTCATGATGTGGATGACGGTTGGTGGCGCGGAGAGACAAATGGCATTGTGGGAAATTTTCCGTCTCTCATTGTCGAGGAATGTGACGAGAATGGAGAACCACTGAGTGTCGTTGAAGAGGACTGGACCCCATCTGGATGCGCTCCTCCGGTCTTCGCTTCACCACCGACCTCACCACCAGGTGCTTCAGATGAAGCTGCGGTAAACGAGAGCAGCGCGGCCCCTCCTCTTGAACCACCACCGCCGCCGCCAGCTGATCTCGGTGACTCGATGGACAGCCAGCCCGACTTTAGCTTTAACCTAGAGTTGACCAGGAACCAGCAGGAGCAATACGGCATGCAGTTCTCGGAGCCCGCCGGCCCACCCGTGCCGCCCCCAGTCACTATTGTTGTTGATGAGGTTGCCAGCGATAATTTCGATGATGAGGACGAAGAAGAGATGCCGCAGCCGATGCCGGTGGTAAAGCCTGTGCCGCCGCCAGTTGAGCTGCCAAGTGCTGGCGAGTGCGGGCTGGGGGTCGCGCAGATTGTGATCACAGCGGCCACCCCGATGGTCGAGGAGCCGGAACTACCGTTCCCGCCCGCAGAGCCGCAGCCGCAGCCGCAACCGCAGATCGACGACGACGACGAGGAGTCGTCGCTGTCCGAGCAAACCGCGGTGTGCGTGCGTCCGCAGCCGACCTCTAGCTCCACAGGCTCGGAGGGCGATTCAACAGGCCCGCACTCTCCACCCGCTGCCCCACGAGCCGGTCGCGCCTCCATCCCCGATGAACTAGAGCCCGCCCAGCTGGCGAGACTCACCGACCTCAAGGAGTCCAACGCCTAA

Protein

MWNVWRTVLEENEKLARARLAAVEVFQQHITDEAKLLRHQKLSSARKFTEGLAQAHKELQMTVGEVDKTKKLYFDEEHTAHDVRDKAKDIEENKPSSSRGSPKHQPPERNELVVDKYWLKKKKGSFFQSITSLQKNSAKVSSRRDQLEEKSTGARNDYILSIAAANAHQNRYFLVDLQTCMQNMESSVYEKVSEYLTLMGRTELLTCSATQHSFGKIRDQAQQLTREYNLQCLYLYYPVLKQHIQYEFEPCDNDPIDTITMEHESVAVTLAQEARRWATRVVREASLVRDANRKISSYQAMRESGHKVDPNEPNGPELEVKMDELRATIRRSEISKAKYEARLECLRVGGAPVDDWLKEIDLLAVQETLPRSSSLLSVRTDASGAADQPSSDSFYDSDNTEGEAAASVAGTSGSGGHQRTTSGSQHEDDHEDEVDAALEIERQRIEQLAGGWDDPTQVNWGESDPEPSTSEPIEPPAPPLYKCTALYSYTAQNPDELSIIENEQLEVVGEGDGDGWLKARNYRGEEGYVPHNYLDVDHDQASSAPGLVSQISFSSVDYTVEGEDADVVQSPDQISVISAPVGKPDEPTKAAEAAQGEAAAVAQGPPKVDLPTLGYCFALYDYEAEACDELNLEEGQIIRVVSRNAHDVDDGWWRGETNGIVGNFPSLIVEECDENGEPLSVVEEDWTPSGCAPPVFASPPTSPPGASDEAAVNESSAAPPLEPPPPPPADLGDSMDSQPDFSFNLELTRNQQEQYGMQFSEPAGPPVPPPVTIVVDEVASDNFDDEDEEEMPQPMPVVKPVPPPVELPSAGECGLGVAQIVITAATPMVEEPELPFPPAEPQPQPQPQIDDDDEESSLSEQTAVCVRPQPTSSSTGSEGDSTGPHSPPAAPRAGRASIPDELEPAQLARLTDLKESNA

Description

Adapter protein that provides a link between vesicular membrane traffic and the actin assembly machinery. Acts together with Cdc42 to stimulate actin nucleation mediated by WASp and the ARP2/3 complex (PubMed:18701694, PubMed:27601635). Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and causes local membrane deformation (PubMed:23761074, PubMed:26686642). Required for normal structure and function of synapses at the neuromuscular junction (PubMed:18701694, PubMed:14980202, PubMed:18498733, PubMed:21464232, PubMed:26686642, PubMed:26567222, PubMed:27601635, PubMed:29568072). Plays a role in synaptic vesicle trafficking (PubMed:29568072). Required for the release of a normal number of synaptic vesicles per action potential (PubMed:26567222).

Subunit

Homodimer (Probable) (PubMed:27601635). Interacts (via SH3 domain 1) with WASp (PubMed:14980202, PubMed:18701694, PubMed:27601635). Interacts (via SH3 domain 1) with shi/dynamin (PubMed:18701694, PubMed:18498733). Interacts (via SH3 domain 2) with Dap160 (PubMed:18701694, PubMed:18498733, PubMed:26686642). Interacts (via F-BAR domain) with SH3PX1 (PubMed:26567222). Interacts (via SH3 domain 2) with Snx16 (PubMed:21464232). Identified in a complex with Syn and Syt1 (PubMed:29568072).

Keywords

Alternative splicing   Cell junction   Cell membrane   Cell projection   Complete proteome   Cytoplasmic vesicle   Endosome   Lipid-binding   Membrane   Reference proteome   Repeat   SH3 domain   Synapse  

Feature

chain  Protein nervous wreck
splice variant  In isoform 3, isoform 4 and isoform 7.

Uniprot

F2Z9J3   F2Z9J4   F2Z9J5   H9IXL3   A0A194Q4X9   A0A194QUY6   + More
A0A212FHJ0   A0A2H1WKM9   A0A2A4JQQ7   A0A182M6T9   A0A182GKU6   A0A182WI63   A0A182R9B1   A0A084WN04   A0A182QAQ5   A0A182N7I7   A0A182PEX6   A0A1S4F8X0   D7EJ83   A0A182V5H8   A0A182YJI0   A0A182JYR8   A0A182UDC0   A0A182X244   A0A182FFG9   A0A182T341   A0A182I6A5   Q0IEP6   A0A182J2H7   W5JVR5   A0A182L436   N6UJW0   A0A1W4X4Q9   A0A1W4XEA4   A0A1W4XFH8   A0A1W4XF75   A0A1B0D741   B0WLT2   A0A0C9RVR1   U4TPJ7   W8B4G9   W8BEK3   W8B4G6   A0A034VUZ4   A0A034VXJ3   A0A2A3EFW9   A0A0M3QW65   A0A1B0G7J7   A0A1B0C2P2   A0A034VVX8   A0A034VWM8   V9IEE5   A0A0Q9XAM2   A0A0Q9XMJ2   A0A0Q9XM53   B4KWM5   A0A088ATW5   A0A1I8PXU3   A0A0L0CNP5   A0A0Q9WUM9   A0A1A9ZC18   A0A0Q9WHZ9   A0A1A9XQZ4   A0A1A9UP20   A0A0P8ZWB8   A0A0Q9WSV5   A0A0P8XW46   A0A0P8XW74   A0A0Q9WHH4   A0A0Q9WSZ6   A0A0M9A852   A0A0P8YIX5   A0A1L8DME3   A0A0P9AMA5   A0A0P8YC42   A0A0A1X6B3   A0A1Y1M0Z7   A0A1I8PXW8   A0A0Q9WUJ2   A0A1Y1M549   B3M8W8   X2JAU8-5   A0A026W4P8   B4LHG5   A0A0L7QSK4   A0A0Q5UHK9   A0A154PA73   X2JAU8-2   X2JAU8-6   A0A0Q5U3S5   E0VZW6   A0A0R1E0A2   A0A0Q5UHN1   A0A0R1DUU2   A0A0Q5U2S9   B4QMQ6   A0A0R1E0C3   B4HKL4   X2JAU8   B4N6Z5  

Pubmed

19121390    26354079    22118469    26483478    24438588    17510324    + More
18362917    19820115    25244985    20920257    23761445    20966253    23537049    24495485    25348373    17994087    18057021    26108605    25830018    28004739    10731132    12537572    14980202    18701694    18498733    21464232    23761074    26686642    26567222    27601635    29568072    24508170    30249741    20566863    17550304   

EMBL

AB600238    BAK22648.1    AB600239    BAK22649.1    AB600240    BAK22650.1    + More
BABH01016014    BABH01016015    BABH01016016    KQ459463    KPJ00593.1    KQ461108    KPJ09129.1    AGBW02008503    OWR53202.1    ODYU01009248    SOQ53526.1    NWSH01000870    PCG73783.1    AXCM01004836    JXUM01014113    JXUM01014114    JXUM01014115    KQ560394    KXJ82642.1    ATLV01024543    KE525352    KFB51598.1    AXCN02000573    AXCN02000574    DS497698    EFA12613.1    APCN01003602    CH477558    CH477317    EAT39001.1    EAT43741.1    ADMH02000276    ETN67155.1    APGK01021192    KB740293    ENN80951.1    AJVK01012324    DS231990    EDS30660.1    GBYB01013050    JAG82817.1    KB630005    KB630385    ERL83334.1    ERL83576.1    GAMC01018369    JAB88186.1    GAMC01018371    JAB88184.1    GAMC01018374    JAB88181.1    GAKP01012718    JAC46234.1    GAKP01012719    JAC46233.1    KZ288255    PBC30683.1    CP012525    ALC43609.1    CCAG010022578    JXJN01024608    JXJN01024609    GAKP01012720    JAC46232.1    GAKP01012717    JAC46235.1    JR041711    AEY59498.1    CH933809    KRG05574.1    KRG05575.1    KRG05569.1    EDW17472.2    KRG05570.1    KRG05572.1    KRG05573.1    KRG05576.1    JRES01000128    KNC33940.1    CH940647    KRF83877.1    KRF83873.1    KRF83874.1    CH902618    KPU78938.1    KRF83876.1    KPU78934.1    KPU78941.1    KRF83875.1    KRF83870.1    KQ435724    KOX78426.1    KPU78935.1    KPU78936.1    KPU78942.1    GFDF01006456    JAV07628.1    KPU78933.1    KPU78939.1    GBXI01007468    JAD06824.1    GEZM01042991    JAV79409.1    KRF83872.1    GEZM01042992    JAV79405.1    EDV41119.2    AE014296    AHN58019.1    KK107419    QOIP01000003    EZA51055.1    RLU24467.1    EDW68495.2    KQ414758    KOC61529.1    CH954178    KQS43370.1    KQ434846    KZC08284.1    KQS43369.1    AAZO01006553    AAZO01006554    DS235854    EEB18922.1    CM000159    KRK00922.1    KQS43364.1    KQS43366.1    KRK00920.1    KRK00923.1    KQS43368.1    CM000363    EDX09810.1    KRK00916.1    CH480815    EDW40817.1    CH964168    EDW80134.2   

Proteomes

UP000005204    UP000053268    UP000053240    UP000007151    UP000218220    UP000075883    + More
UP000069940    UP000249989    UP000075920    UP000075900    UP000030765    UP000075886    UP000075884    UP000075885    UP000007266    UP000075903    UP000076408    UP000075881    UP000075902    UP000076407    UP000069272    UP000075901    UP000075840    UP000008820    UP000075880    UP000000673    UP000075882    UP000019118    UP000192223    UP000092462    UP000002320    UP000030742    UP000242457    UP000092553    UP000092444    UP000092460    UP000009192    UP000005203    UP000095300    UP000037069    UP000008792    UP000092445    UP000092443    UP000078200    UP000007801    UP000053105    UP000000803    UP000053097    UP000279307    UP000053825    UP000008711    UP000076502    UP000009046    UP000002282    UP000000304    UP000001292    UP000007798   

PRIDE

F2Z9J3    F2Z9J4    F2Z9J5    H9IXL3   

Pfam

PF00611   FCH        + More
PF00018   SH3_1
PF14604   SH3_9
PF07653   SH3_2
PF10513   EPL1

Interpro

IPR035460   FCHSD_SH3_1        + More
IPR036028   SH3-like_dom_sf       
IPR001452   SH3_domain       
IPR031160   F_BAR       
IPR001060   FCH_dom       
IPR027267   AH/BAR_dom_sf       
IPR013083   Znf_RING/FYVE/PHD       
IPR001965   Znf_PHD       
IPR019787   Znf_PHD-finger       
IPR011011   Znf_FYVE_PHD       
IPR034732   EPHD       
IPR019786   Zinc_finger_PHD-type_CS       
IPR019542   Enhancer_polycomb-like_N       

SUPFAM

SSF103657   SSF103657        + More
SSF50044   SSF50044       
SSF57903   SSF57903       

Gene 3D

1.20.1270.60     3.30.40.10    

CDD

cd11761  SH3_FCHSD_1

ProteinModelPortal

F2Z9J3   F2Z9J4   F2Z9J5   H9IXL3   A0A194Q4X9   A0A194QUY6   + More
A0A212FHJ0   A0A2H1WKM9   A0A2A4JQQ7   A0A182M6T9   A0A182GKU6   A0A182WI63   A0A182R9B1   A0A084WN04   A0A182QAQ5   A0A182N7I7   A0A182PEX6   A0A1S4F8X0   D7EJ83   A0A182V5H8   A0A182YJI0   A0A182JYR8   A0A182UDC0   A0A182X244   A0A182FFG9   A0A182T341   A0A182I6A5   Q0IEP6   A0A182J2H7   W5JVR5   A0A182L436   N6UJW0   A0A1W4X4Q9   A0A1W4XEA4   A0A1W4XFH8   A0A1W4XF75   A0A1B0D741   B0WLT2   A0A0C9RVR1   U4TPJ7   W8B4G9   W8BEK3   W8B4G6   A0A034VUZ4   A0A034VXJ3   A0A2A3EFW9   A0A0M3QW65   A0A1B0G7J7   A0A1B0C2P2   A0A034VVX8   A0A034VWM8   V9IEE5   A0A0Q9XAM2   A0A0Q9XMJ2   A0A0Q9XM53   B4KWM5   A0A088ATW5   A0A1I8PXU3   A0A0L0CNP5   A0A0Q9WUM9   A0A1A9ZC18   A0A0Q9WHZ9   A0A1A9XQZ4   A0A1A9UP20   A0A0P8ZWB8   A0A0Q9WSV5   A0A0P8XW46   A0A0P8XW74   A0A0Q9WHH4   A0A0Q9WSZ6   A0A0M9A852   A0A0P8YIX5   A0A1L8DME3   A0A0P9AMA5   A0A0P8YC42   A0A0A1X6B3   A0A1Y1M0Z7   A0A1I8PXW8   A0A0Q9WUJ2   A0A1Y1M549   B3M8W8   X2JAU8-5   A0A026W4P8   B4LHG5   A0A0L7QSK4   A0A0Q5UHK9   A0A154PA73   X2JAU8-2   X2JAU8-6   A0A0Q5U3S5   E0VZW6   A0A0R1E0A2   A0A0Q5UHN1   A0A0R1DUU2   A0A0Q5U2S9   B4QMQ6   A0A0R1E0C3   B4HKL4   X2JAU8   B4N6Z5  

PDB

2DL7     E-value=2.02601e-10,     Score=161

KEGG

100616528    K20125 F-BAR and double SH3 domains protein | (RefSeq) nwk, Bmnwk; nervous wreck

GO

GO:0046872   GO:0031594   GO:0055037   GO:0007274   GO:0030833   GO:0044803   GO:2000601   GO:0043325   GO:0045886   GO:0005546   GO:0001881   GO:0042734   GO:0005543   GO:0030424   GO:0008582   GO:0008021   GO:0030054   GO:0005737   GO:0005515   GO:0007264   GO:0007165   GO:0016020   GO:0043565   GO:0043401   GO:0003677   GO:0003707   GO:0007169   GO:0016021  

Subcellular location

Endomembrane system   Detected at presynaptic axon terminals at the neuromuscular junction (PubMed:14980202, PubMed:18701694, PubMed:21464232, PubMed:26686642, PubMed:26567222, PubMed:27601635, PubMed:29568072). Colocalizes with Cdc42 and Rab11 (PubMed:18701694, PubMed:21464232).   With evidence from 3 publications.
Cell junction   Detected at presynaptic axon terminals at the neuromuscular junction (PubMed:14980202, PubMed:18701694, PubMed:21464232, PubMed:26686642, PubMed:26567222, PubMed:27601635, PubMed:29568072). Colocalizes with Cdc42 and Rab11 (PubMed:18701694, PubMed:21464232).   With evidence from 3 publications.
Synapse   Detected at presynaptic axon terminals at the neuromuscular junction (PubMed:14980202, PubMed:18701694, PubMed:21464232, PubMed:26686642, PubMed:26567222, PubMed:27601635, PubMed:29568072). Colocalizes with Cdc42 and Rab11 (PubMed:18701694, PubMed:21464232).   With evidence from 3 publications.
Cell projection   Detected at presynaptic axon terminals at the neuromuscular junction (PubMed:14980202, PubMed:18701694, PubMed:21464232, PubMed:26686642, PubMed:26567222, PubMed:27601635, PubMed:29568072). Colocalizes with Cdc42 and Rab11 (PubMed:18701694, PubMed:21464232).   With evidence from 3 publications.
Axon   Detected at presynaptic axon terminals at the neuromuscular junction (PubMed:14980202, PubMed:18701694, PubMed:21464232, PubMed:26686642, PubMed:26567222, PubMed:27601635, PubMed:29568072). Colocalizes with Cdc42 and Rab11 (PubMed:18701694, PubMed:21464232).   With evidence from 3 publications.
Presynaptic cell membrane   Detected at presynaptic axon terminals at the neuromuscular junction (PubMed:14980202, PubMed:18701694, PubMed:21464232, PubMed:26686642, PubMed:26567222, PubMed:27601635, PubMed:29568072). Colocalizes with Cdc42 and Rab11 (PubMed:18701694, PubMed:21464232).   With evidence from 3 publications.
Cytoplasmic vesicle   Detected at presynaptic axon terminals at the neuromuscular junction (PubMed:14980202, PubMed:18701694, PubMed:21464232, PubMed:26686642, PubMed:26567222, PubMed:27601635, PubMed:29568072). Colocalizes with Cdc42 and Rab11 (PubMed:18701694, PubMed:21464232).   With evidence from 3 publications.
Secretory vesicle   Detected at presynaptic axon terminals at the neuromuscular junction (PubMed:14980202, PubMed:18701694, PubMed:21464232, PubMed:26686642, PubMed:26567222, PubMed:27601635, PubMed:29568072). Colocalizes with Cdc42 and Rab11 (PubMed:18701694, PubMed:21464232).   With evidence from 3 publications.
Synaptic vesicle   Detected at presynaptic axon terminals at the neuromuscular junction (PubMed:14980202, PubMed:18701694, PubMed:21464232, PubMed:26686642, PubMed:26567222, PubMed:27601635, PubMed:29568072). Colocalizes with Cdc42 and Rab11 (PubMed:18701694, PubMed:21464232).   With evidence from 3 publications.
Recycling endosome   Detected at presynaptic axon terminals at the neuromuscular junction (PubMed:14980202, PubMed:18701694, PubMed:21464232, PubMed:26686642, PubMed:26567222, PubMed:27601635, PubMed:29568072). Colocalizes with Cdc42 and Rab11 (PubMed:18701694, PubMed:21464232).   With evidence from 3 publications.

Length:

918

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00018

Exp number, first 60 AAs:

0

Total prob of N-in:

0.00004

outside

1  -  918

 

 

Pi

23.896096

Theta

18.295427

Tajima's D

-1.701202

CLR

0.052267

CSRT

0.0326483675816209

Interpretation

Uncertain