Gene

KWMTBOMO00154  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA002149

Annotation

PREDICTED:_abhydrolase_domain-containing_protein_16A_isoform_X2_[Bombyx_mori]

Location

Bomo_Chr1(+):4810454-4817783      View in EpiBrowser

Full name

Phosphatidylserine lipase ABHD16A      

Alternative Name

Alpha/beta hydrolase domain-containing protein 16A
HLA-B-associated transcript 5
Monoacylglycerol lipase ABHD16A
Protein G5
HLA-B-associated transcript 5 homolog

Location in the cell

PlasmaMembrane   Reliability : 3.761

CDS

ATGGGGTCTACTGTGGCTAGAATGAGGCGTATGTGGCGATGCATGTTTTCTCCTCGACTCTACAAGATTTACTTCGATGGACGACAAGAGGATCCGTATCGCCCGGTCGGAGTTGAAAAGATTGGTGAAAGGACCCTTGCAACGGCCTATACCGCTATCAGAATCGGTTATTATGCAACTCCATTGATCTGCCTATACATAATCCAACGTGGCTTTCTTTCAATGGAAGAAGTAAAAACTTTAATGCGCTTCTTCGGAGGCATCGGCTGTATAGCTACTGTTTTCTTCGTAATGCGAGCATATGGAAGATCTTACAGTCCGAAATATTTGAAATTCATTGACACTTTGGACTCTCCCATGGACGACAAGAATGCGTATCTGAAGGCGATCAGGAAATACGATTTCGACTTCTCCGCTTGGCCTGTAACCTTTACCGCTGATCCCGAAGAAAGGCAAGAAAGCTGGCTGCAGAACCACCCTTTTGCGAAATGCGCGAACATGGACTTACCTGTTTATCAGAGGGTTATAATCCAAATTTTGGCTTTTATTGCGGCCCATACCTTCGGTATTCGTCTCATCTACCCAGGATCATTGTTCTTGATACATAATCTGTTATGGAGCGGTCTGCTCAGTGGACGCACTCAACTCGTCGAGTACTACAACGGGGAGCGCGCCAAGATCTGCACTTCCGACGGAAACCATATCGAGACAATGTTCGTTGAAAGCCGACACAAGTCATTCAAAGGTAGGACCCTGATCATCTGCTGCGAGGGAAACTCTGGTTACTATGAGATTGGCGTTATGACGACACCGGTCAAAGGTGGATTTTCCGTTCTCGGCTGGAACCACCCTGGCTTCGGCGGAAGCACTGGAACACCATTCCCGGATCAGGAAAAGCGTGCCATGGACGCTGTAATCCAGTATGCCATTTACGAGCTTTTCTTTGCCCCCGAAGACATAGTGATTTACGGATGGAGCATCGGTGGCTTCACAGCCGCATATGGTGCCGCTAACTATCCGATTAAAGCATTGATTCTGGACGCGACCTTCGATGACCTTTTGCCGCTGGCCAAGAATCAGATGCCTTCCTCGTGGTCTCGTCTGGTGAAGGAAGTAATTCGCTCTTATGTGGACCTGAACATTGGCGAACTAGTCAACCAGTACGAAGGACCTCTTCAATTGATCAGGAGGGGTGACGATGAGATTATTTGCTTGAGGTCTGGCGACATCACCACTAATCGTTGCAACAACCTAGCGTTGAAAGTAATAAGCCATCGCCATCCGAAACTGGCCAAAGATGAGGACTATATGAGGCTTTTCGTGAACTGCGTTGCCATGACCGAGAACGAGCGCGTGGAGGCCTATGAAACTGATATCACTTCCAACGAACGTACTGTGCTGGATGTGGTGTCGCTGTACATGCGTGACTTCGCTTCGAACCACTGCACCGCCCTGCCGGAGACCCACTTTGACATGATCATGGAGTTGCTCGAGGTAGCCCCAGGTGTGCAGCGAGAACTTAATTCTTAA

Protein

MGSTVARMRRMWRCMFSPRLYKIYFDGRQEDPYRPVGVEKIGERTLATAYTAIRIGYYATPLICLYIIQRGFLSMEEVKTLMRFFGGIGCIATVFFVMRAYGRSYSPKYLKFIDTLDSPMDDKNAYLKAIRKYDFDFSAWPVTFTADPEERQESWLQNHPFAKCANMDLPVYQRVIIQILAFIAAHTFGIRLIYPGSLFLIHNLLWSGLLSGRTQLVEYYNGERAKICTSDGNHIETMFVESRHKSFKGRTLIICCEGNSGYYEIGVMTTPVKGGFSVLGWNHPGFGGSTGTPFPDQEKRAMDAVIQYAIYELFFAPEDIVIYGWSIGGFTAAYGAANYPIKALILDATFDDLLPLAKNQMPSSWSRLVKEVIRSYVDLNIGELVNQYEGPLQLIRRGDDEIICLRSGDITTNRCNNLALKVISHRHPKLAKDEDYMRLFVNCVAMTENERVEAYETDITSNERTVLDVVSLYMRDFASNHCTALPETHFDMIMELLEVAPGVQRELNS

Description

Phosphatidylserine (PS) lipase that mediates the hydrolysis of phosphatidylserine to generate lysophosphatidylserine (LPS) (By similarity). LPS constitutes a class of signaling lipids that regulates immunological and neurological processes (By similarity). Has no activity towards diacylglycerol, triacylglycerol or lysophosphatidylserine lipase (PubMed:25290914). Also has monoacylglycerol lipase activity, with preference for 1-(9Z,12Z-octadecadienoyl)-glycerol (1-LG) and 2-glyceryl-15-deoxy-Delta(12,14)-prostaglandin J2 (15d-PGJ(2)-G) (PubMed:25290914).
Phosphatidylserine (PS) lipase that mediates the hydrolysis of phosphatidylserine to generate lysophosphatidylserine (LPS). LPS constitutes a class of signaling lipids that regulates immunological and neurological processes (By similarity). Has no activity towards diacylglycerol, triacylglycerol or lysophosphatidylserine lipase (By similarity). Also has monoacylglycerol lipase activity, with preference for 1-(9Z,12Z-octadecadienoyl)-glycerol (1-LG) and 2-glyceryl-15-deoxy-Delta(12,14)-prostaglandin J2 (15d-PGJ(2)-G) (By similarity).

Catalytic Activity

1-heptadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoserine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + 1-heptadecanoyl-sn-glycero-3-phosphoserine + H(+)
1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + H2O = (9Z)-octadecenoate + 1-hexadecanoyl-sn-glycero-3-phospho-L-serine + H(+)
1-octadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoserine + H2O = (9Z,12Z)-octadecadienoate + 1-octadecanoyl-sn-glycero-3-phosphoserine + H(+)
1-heptadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + 1-heptadecanoyl-sn-glycero-3-phosphocholine + H(+)
1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-glycerol + H2O = (9Z)-octadecenoate + 1-hexadecanoyl-sn-glycero-3-phospho-glycerol + H(+)
1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1D-myo-inositol) + H2O = (9Z)-octadecenoate + 1-hexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol) + H(+)
1-heptadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + 1-heptadecanoyl-sn-glycero-3-phosphoethanolamine + H(+)
1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + H2O = (9Z)-octadecenoate + 1-hexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) + H(+)
Hydrolyzes glycerol monoesters of long-chain fatty acids.
1-tetradecanoylglycerol + H2O = glycerol + H(+) + tetradecanoate
2-hexadecanoylglycerol + H2O = glycerol + H(+) + hexadecanoate
1-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + glycerol + H(+)
2-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + glycerol + H(+)
2-(9Z,12Z-octadecadienoyl)-glycerol + H2O = (9Z,12Z)-octadecadienoate + glycerol + H(+)
1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycerol + H(+)
2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycerol + H(+)
H2O + prostaglandin D2-1-glycerol ester = glycerol + H(+) + prostaglandin D2
11-oxo-5Z,9,12E,14E-prostatetraenoate + H2O = 15-deoxy-Delta(12,14)-prostaglandin J2 + glycerol + H(+)
1-(9Z,12Z-octadecadienoyl)-glycerol + H2O = (9Z,12Z)-octadecadienoate + glycerol + H(+)

Biophysicochemical Properties

6.9 uM for 1-(9Z,12Z-octadecadienoyl)-glycerol (1-LG) (in absence of BSA)
137.8 uM for 1-(9Z,12Z-octadecadienoyl)-glycerol (1-LG) (in presence of BSA)
20.9 uM for 2-glyceryl-15-deoxy-Delta(12,14)-prostaglandin J2 (15d-PGJ(2)-G) (in presence of BSA)

Similarity

Belongs to the AB hydrolase superfamily. ABHD16 family.

Keywords

Alternative splicing   Complete proteome   Direct protein sequencing   Hydrolase   Lipid metabolism   Membrane   Reference proteome   Transmembrane   Transmembrane helix  

Feature

chain  Phosphatidylserine lipase ABHD16A
splice variant  In isoform 2.

Uniprot

H9IY16   A0A2A4JP56   S4P7Z9   A0A212FHI4   A0A194QZV8   A0A194QAZ2   + More
A0A0L7L516   A0A1Q3FG74   A0A1Q3FGC9   A0A1W4XSM5   Q16QP9   D6WGV7   A0A2M4BKC1   A0A2M4BK96   W5J2I1   E0VXH4   A0A1B6E388   A0A2M4AH09   A0A1Y1KAP3   A0A1Y1KDS7   A0A1Q3FGC0   A0A1Y1KIB6   A0A1Y1KFW4   A0A182WAM5   A0A1Q3FGG0   A0A1B6IXS9   A0A2M4AH87   A0A182K8R9   A0A182N7U9   A0A182IW07   A0A182FGS7   A0A182YNM9   A0A182R2M5   A0A1B6EY25   A0A1S3D365   A0A182GWC6   A0A182V6U8   Q7Q7D8   A0A182SUJ1   A0A1B6KEP0   A0A1B6G5I4   A0A0K8S3N3   H9GPA2   A0A1B0GJN5   A0A1L8D930   A0A1L8D9G6   A0A2Y9EC41   A0A182M2V6   A0A1L8D9D5   A0A0L0BR43   A0A182K6T9   A0A1S3AMA4   A0A2Y9HMI3   A0A182QVM4   A0A340XDT7   A0A387LA74   G3VIK5   F7IA46   U3E3N7   A0A084WEW7   A0A341AV91   A0A2D0R307   F6TLV9   A0A1W4YGX4   A0A3B3T8W4   A0A2Y9MXJ3   U4UMK9   G3T3U4   A0A0K8TQ26   A0A034WKB2   M3W2B0   F7BM46   A0A2K5JMS4   E2RQ56   A0A2K5RCX9   H0X2C4   A0A2Y9EY17   A0A3P4RQY8   A0A0T6BCS4   A0A1U7RE48   A0A1U7SAF5   G3R3B4   A0A1U9X777   O95870   K7J687   G1PDP2   A0A151NC84   H0VHQ3   A0A2Y9K495   A0A287AWB5   A0A2K6TF19   Q5R6S0   Q6MG55   A0A3B4CU18   A0A0G2JJD3   A0A2U3WZY6   A0A1I8M428   A0A2K5CUT2   B9TSQ5  

EC Number

3.1.-.-

Pubmed

19121390    23622113    22118469    26354079    26227816    17510324    + More
18362917    19820115    20920257    23761445    20566863    28004739    25244985    26483478    12364791    14747013    17210077    25727380    21881562    26108605    21709235    25243066    24438588    17495919    29240929    23537049    26369729    25348373    17975172    19892987    16341006    15498874    14702039    14656967    14574404    15489334    12665801    24275569    25290914    20075255    21993624    22293439    15060004    11181995    25315136   

EMBL

BABH01016007    BABH01016008    BABH01016009    BABH01016010    NWSH01000870    PCG73781.1    + More
GAIX01007477    JAA85083.1    AGBW02008503    OWR53203.1    KQ461108    KPJ09126.1    KQ459463    KPJ00591.1    JTDY01002984    KOB70369.1    GFDL01008543    JAV26502.1    GFDL01008493    JAV26552.1    CH477738    EAT36728.1    KQ971321    EFA00151.1    GGFJ01004356    MBW53497.1    GGFJ01004355    MBW53496.1    ADMH02002185    ETN57941.1    DS235832    EEB18080.1    GEDC01006403    GEDC01004923    JAS30895.1    JAS32375.1    GGFK01006752    MBW40073.1    GEZM01087746    JAV58509.1    GEZM01087745    JAV58511.1    GFDL01008445    JAV26600.1    GEZM01087747    JAV58507.1    GEZM01087748    JAV58505.1    GFDL01008405    JAV26640.1    GECU01036843    GECU01015972    JAS70863.1    JAS91734.1    GGFK01006829    MBW40150.1    GECZ01026881    GECZ01008758    GECZ01000073    JAS42888.1    JAS61011.1    JAS69696.1    JXUM01004304    KQ560172    KXJ84009.1    AAAB01008960    EAA11786.4    GEBQ01030049    JAT09928.1    GECZ01012087    JAS57682.1    GBKC01001623    GBKD01000238    JAG44447.1    JAG47380.1    AAWZ02030713    AJWK01021990    GFDF01011088    JAV02996.1    GFDF01011087    JAV02997.1    AXCM01001699    GFDF01011089    JAV02995.1    JRES01001494    KNC22481.1    AXCN02000589    AB989434    BBG43791.1    AEFK01182230    AEFK01182231    GAMQ01005052    JAB36799.1    ATLV01023260    KE525341    KFB48761.1    KB632385    ERL94332.1    GDAI01001111    JAI16492.1    GAKP01004377    JAC54575.1    AANG04003556    AAEX03008213    AAEX03008214    AAQR03060250    AAQR03060251    CYRY02043120    VCX37287.1    LJIG01001804    KRT85136.1    CABD030044187    CABD030044188    CABD030044189    KY500136    KY500137    KY500138    KY500139    KY500140    KY500141    KY500142    KY500143    AQY76644.1    AF193047    AK001207    AK023194    AK297712    AF129756    BA000025    AL662899    AL670886    AL805934    BX248244    BX511262    CR354443    CR753842    CR759761    CR759787    BC031839    AAPE02050100    AKHW03003475    KYO34428.1    AAKN02021589    AEMK02000053    CR860415    BX883045    BC091227    CH471081    EAX03483.1    EU282343    ABX82812.1   

Proteomes

UP000005204    UP000218220    UP000007151    UP000053240    UP000053268    UP000037510    + More
UP000192223    UP000008820    UP000007266    UP000000673    UP000009046    UP000075920    UP000075881    UP000075884    UP000075880    UP000069272    UP000076408    UP000075900    UP000079169    UP000069940    UP000249989    UP000075903    UP000007062    UP000075901    UP000001646    UP000092461    UP000248480    UP000075883    UP000037069    UP000079721    UP000248481    UP000075886    UP000265300    UP000007648    UP000008225    UP000030765    UP000252040    UP000221080    UP000002280    UP000192224    UP000261540    UP000248483    UP000030742    UP000007646    UP000011712    UP000002281    UP000233080    UP000002254    UP000233040    UP000005225    UP000248484    UP000189706    UP000189705    UP000001519    UP000005640    UP000002358    UP000001074    UP000050525    UP000005447    UP000248482    UP000008227    UP000233220    UP000001595    UP000002494    UP000261440    UP000245340    UP000095301    UP000233020   

PRIDE

H9IY16    O95870    Q6MG55   

Pfam

PF03109   ABC1        + More
PF00561   Abhydrolase_1
PF12697   Abhydrolase_6
PF12146   Hydrolase_4

Interpro

IPR034646   ADCK3_UbiB        + More
IPR000073   AB_hydrolase_1       
IPR004147   UbiB_dom       
IPR029058   AB_hydrolase       
IPR011009   Kinase-like_dom_sf       
IPR026604   ABHD16A       
IPR022742   Hydrolase_4       

SUPFAM

SSF56112   SSF56112        + More
SSF53474   SSF53474       

Gene 3D

3.40.50.1820    

CDD

cd13970  ABC1_ADCK3

ProteinModelPortal

H9IY16   A0A2A4JP56   S4P7Z9   A0A212FHI4   A0A194QZV8   A0A194QAZ2   + More
A0A0L7L516   A0A1Q3FG74   A0A1Q3FGC9   A0A1W4XSM5   Q16QP9   D6WGV7   A0A2M4BKC1   A0A2M4BK96   W5J2I1   E0VXH4   A0A1B6E388   A0A2M4AH09   A0A1Y1KAP3   A0A1Y1KDS7   A0A1Q3FGC0   A0A1Y1KIB6   A0A1Y1KFW4   A0A182WAM5   A0A1Q3FGG0   A0A1B6IXS9   A0A2M4AH87   A0A182K8R9   A0A182N7U9   A0A182IW07   A0A182FGS7   A0A182YNM9   A0A182R2M5   A0A1B6EY25   A0A1S3D365   A0A182GWC6   A0A182V6U8   Q7Q7D8   A0A182SUJ1   A0A1B6KEP0   A0A1B6G5I4   A0A0K8S3N3   H9GPA2   A0A1B0GJN5   A0A1L8D930   A0A1L8D9G6   A0A2Y9EC41   A0A182M2V6   A0A1L8D9D5   A0A0L0BR43   A0A182K6T9   A0A1S3AMA4   A0A2Y9HMI3   A0A182QVM4   A0A340XDT7   A0A387LA74   G3VIK5   F7IA46   U3E3N7   A0A084WEW7   A0A341AV91   A0A2D0R307   F6TLV9   A0A1W4YGX4   A0A3B3T8W4   A0A2Y9MXJ3   U4UMK9   G3T3U4   A0A0K8TQ26   A0A034WKB2   M3W2B0   F7BM46   A0A2K5JMS4   E2RQ56   A0A2K5RCX9   H0X2C4   A0A2Y9EY17   A0A3P4RQY8   A0A0T6BCS4   A0A1U7RE48   A0A1U7SAF5   G3R3B4   A0A1U9X777   O95870   K7J687   G1PDP2   A0A151NC84   H0VHQ3   A0A2Y9K495   A0A287AWB5   A0A2K6TF19   Q5R6S0   Q6MG55   A0A3B4CU18   A0A0G2JJD3   A0A2U3WZY6   A0A1I8M428   A0A2K5CUT2   B9TSQ5  

KEGG

101739335    no KO assigned | (RefSeq) abhydrolase domain-containing protein 16A

GO

GO:0016021   GO:0016787   GO:0047372   GO:1905344   GO:0052651  

PANTHER

PTHR43851   PTHR12277:SF54  

Subcellular location

Membrane  

Length:

509

Number of predicted TMHs:

3

Exp number of AAs in TMHs:

64.51089

Exp number, first 60 AAs:

9.81683

Total prob of N-in:

0.97910

inside

1  -  49

TMhelix

50  -  72

outside

73  -  81

TMhelix

82  -  101

inside

102  -  174

TMhelix

175  -  194

outside

195  -  509

 

 

Pi

8.136164

Theta

15.405641

Tajima's D

-1.292871

CLR

0.045679

CSRT

0.0889455527223639

Interpretation

Uncertain