Gene

KWMTBOMO00033  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA002085

Annotation

CYP303A1_[Helicoverpa_armigera]

Location

Bomo_Chr1(+):1058706-1068196      View in EpiBrowser

Full name

Probable cytochrome P450 303a1       + More
Methyl farnesoate epoxidase      

Alternative Name

CYPCCCIIIA1
Cytochrome P450 CYP15A1

Location in the cell

Cytoplasmic   Reliability : 1.403 Mitochondrial   Reliability : 1.467

CDS

ATGTGGCTGGCAGCGCTAGCGGTTCTGTTAGCGGTGTGCTTGTATCTCTTTTTGGATACCCTCAAACCGAGAAAATTCCCTCCCGGCCCGAAATGGACACCGATTCTTGGCTGCGCAAAGGAAGTTTACAAGCTTCGAGAAAAAACCGGCTACCTGTACAAAGCCGTTAGAGAATTATCTCTGACCTACTGCAAGGAGACTCCAGTACTAGGTTTGAGAATAGGGAAAGACCGAATCGTGATGGTGAATAGCTTGGAAGCAAACAAAGAGATGTTATTCAATGAGGACATCGACGGAAGACCGAAGGGAATCTTTTATCAAACCAGAACGTGGGGAGAACGACGAGGTGTCTTGTTAACAGACGGGGAACTGTGGAAGGAGCAGAGGCGCTTCCTCATCAAGCACCTTAAAGAATTCGGCTTTGGCAGATCCGGTATGGGCGAGACAGCGAAACTGGAAGCCGAGCACATAGTCATCGACGTAATGCACATGATCGGCGACAGGGGATCAGCCGTTATACAAATGCACAACTTCTTCAACGTTTATATTCTGAATACTCTATGGACGATGATGGCCGGGAATAGGTACAATCCAAGCGACCCACAGATGAAGATCCTTCAAAGCATGCTGTTCGATTTGTTCGCAGCCGTCGACATGGTCGGTACAGCCTTCAGTCACTTCCCGATATTGAGCATCGTAGCACCGACCTTATCCGGTTATAGAAATTTCATTAAAACGCATAAGAGAATCTGGAAGTTCTTGAGAGAAGAACTAGCGAGACATAAAGACAGTTTTCAGCCAGATAAAGAGGATAAGGACTTTATGGATGTGTACATAAGGGCTTTGCGTGAGCACGGAGAAGTGAACACGTATTCCGAGGGACAGCTCGTGGCCATGTGCATGGACATGTTCATGGCGGGCACGGAGACGACCAGCAAGAGTATGAGCTTCTGCTTCAGCTATCTCGTTAGAGAGCAGGAAGTCCAAAGGAAAGCTCAGGAGGAAATTGACAGGGTTGTAGGAAAAGATCGGGTACCGAGCGTGAACGATAGGCCTAACATGCCCTATAACGAAGCAATAGTCCATGAGTGTGTCAGACACTTTATGGGACGTACCTTCGGTGTACCGCATCGGGCTCTACGAGACACCACTTTAGCTGGATGCCATATACCTGAGGACACAATGGTAGTCAGTAACTACACGAACATTCTACTTGACGAGAACTACTTCCCGGAGCCGTATTCCTTCAAGCCTGAGAGGTTCCTGGTAAACGGGAGGGTCAGTCTGCCTGACCACTACTTTCCCTTCGGTCTTGCGAAGCATCGCTGCATGGGCGATGCATTAGCCAAGTGCAATATCTTCGTTTTCACTACTACAATGCTGCAGAGGTTCTCGCTGGTGCCTGTACCGGGGGAAGGTCTGCCGTCCTTGGATCATATGGACGGAGCCACTCCTTCAGCAGCGCCGTTTAAGGCTTTAGTTATACCACGAATTTGA

Protein

MWLAALAVLLAVCLYLFLDTLKPRKFPPGPKWTPILGCAKEVYKLREKTGYLYKAVRELSLTYCKETPVLGLRIGKDRIVMVNSLEANKEMLFNEDIDGRPKGIFYQTRTWGERRGVLLTDGELWKEQRRFLIKHLKEFGFGRSGMGETAKLEAEHIVIDVMHMIGDRGSAVIQMHNFFNVYILNTLWTMMAGNRYNPSDPQMKILQSMLFDLFAAVDMVGTAFSHFPILSIVAPTLSGYRNFIKTHKRIWKFLREELARHKDSFQPDKEDKDFMDVYIRALREHGEVNTYSEGQLVAMCMDMFMAGTETTSKSMSFCFSYLVREQEVQRKAQEEIDRVVGKDRVPSVNDRPNMPYNEAIVHECVRHFMGRTFGVPHRALRDTTLAGCHIPEDTMVVSNYTNILLDENYFPEPYSFKPERFLVNGRVSLPDHYFPFGLAKHRCMGDALAKCNIFVFTTTMLQRFSLVPVPGEGLPSLDHMDGATPSAAPFKALVIPRI

Description

May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.
Catalyzes the conversion of methyl farnesoate to juvenile hormone III acid (methyl (2E,6E)-(10R)-10,11-epoxy-3,7,11-trimethyl-2,6-dodecadienoate) in juvenile hormone biosynthesis.

Catalytic Activity

methyl (2E,6E)-farnesoate + O2 + reduced [NADPH--hemoprotein reductase] = H(+) + H2O + juvenile hormone III + oxidized [NADPH--hemoprotein reductase]

Cofactor

heme

Biophysicochemical Properties

380 uM for methyl (2E,6E)-farnesoate (with purified enzyme)
26 uM for methyl (2E,6E)-farnesoate (with recombinant enzyme expressed in E.coli)

Miscellaneous

The enzyme is present in all insects, except in lepidoptera (moths and butterflies), and is specific for methyl farnesoate. Lepidoptera contain the farnesoate epoxidase, which is specific for farnesoate.

Similarity

Belongs to the cytochrome P450 family.

Keywords

Complete proteome   Endoplasmic reticulum   Heme   Iron   Membrane   Metal-binding   Microsome   Monooxygenase   Oxidoreductase   Reference proteome   Glycoprotein   Signal  

Feature

chain  Probable cytochrome P450 303a1

Uniprot

H9IXV2   A0A0K0TQ03   A0A0K0TQ10   A0A0K0TQ19   A0A2H1VQG3   A0A2A4JH06   + More
A0A194QUP5   A0A194PVH1   A0A0L7LAF8   A0A067QWR9   A0A2J7R878   E0VZW4   A0A0A9X2T3   A0A146M3L6   A0A2H4BKC1   R4G5L1   A0A182UYS2   B0WPM4   A0A182XH82   A0A182KYW1   A0A182HR04   A0A182JVQ4   A0A2H8TP40   J9JSS1   A0A182PTI7   Q7Q0Y0   A0A2D1GSE0   Q16MY7   A0A182S4N6   A0A1J1IJF4   A0A139WKQ2   A0A1B0D403   A0A1W4WY09   A0A182G047   A0A182U1F1   A0A0K0LB78   A0A195CB21   W5JGC0   A0A0L7QSC6   A0A0L0BZA4   A0A1A9XVT2   A0A1B0ANX1   A0A151X6S6   A0A195EGE3   A0A1B0A3X4   A0A1I8N5X7   A0A1B0FH35   A0A195FFS5   B4LTE1   A0A0M3QTZ6   A0A1Q1NKZ1   B4KI15   K4JN70   A0A1A9UQZ8   A0A3B0K0V2   B4JAC4   A0A1I8PM70   A0A1A9WNZ4   B4GQM5   B4MZZ0   Q29NL9   R9UDV8   C4PAT7   A0A151I3Z7   A0A1W4VJM7   B3N654   B4Q6Z4   B4NXQ1   X2JA13   Q9V399   B4I210   A0A0K8U0Y7   B3MJP5   A0A182YQN3   A0A1Y1KV34   A0A336LKB5   A0A158NT15   A0A182M230   U4UI40   A0A2P8XTM3   E2AZS9   E2AZS8   A0A3Q0IZM7   A0A1W6L1Q3   A0A0K8VKY7   A0A0M9A6L7   N6SXQ1   C7SHT4   A0A2A3E0Q3   Q6R7M4   A0A1Y1L2U8   D6WFY6   A0A0F7R7X8  

EC Number

1.14.-.-
1.14.14.127

Pubmed

19121390    26174024    28756777    26354079    26227816    24845553    + More
20566863    25401762    26823975    20966253    12364791    17510324    18362917    19820115    20920257    23761445    26108605    25315136    17994087    28092127    24324548    15632085    22936249    17550304    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    10471707    25244985    28004739    21347285    23537049    29403074    20798317    15024118    25921675   

EMBL

BABH01015663    BABH01015664    BABH01015665    BABH01015666    BABH01015667    KR709083    + More
AKR53659.1    KR709085    AKR53661.1    KR709084    KZ149922    AKR53660.1    PZC77682.1    ODYU01003657    SOQ42702.1    NWSH01001488    PCG71099.1    KQ461108    KPJ09044.1    KQ459593    KPI96759.1    JTDY01002069    KOB72186.1    KK853156    KDR10510.1    NEVH01006723    PNF37031.1    DS235854    EEB18920.1    GBHO01032186    GBHO01032184    JAG11418.1    JAG11420.1    GDHC01004276    JAQ14353.1    KF984041    AHJ59374.1    ACPB03000224    GAHY01000435    JAA77075.1    DS232027    EDS32427.1    APCN01001629    GFXV01003984    MBW15789.1    ABLF02036361    AAAB01008980    EAA13781.4    MF471376    ATN95982.1    CH477845    EAT35703.1    CVRI01000054    CRL00375.1    KQ971328    KYB28395.1    AJVK01011122    AJVK01011123    KM217008    AIW79971.1    KQ978023    KYM98059.1    ADMH02001289    ETN63136.1    KQ414758    KOC61548.1    JRES01001119    KNC25358.1    JXJN01001070    KQ982476    KYQ56062.1    KQ978957    KYN27308.1    CCAG010021907    KQ981636    KYN38874.1    CH940649    EDW63911.1    CP012523    ALC39749.1    KX660712    AQM57051.1    CH933807    EDW11297.1    JX876491    AFU86421.1    OUUW01000006    SPP81470.1    CH916368    EDW03795.1    CH479187    EDW39897.1    CH963920    EDW77925.1    CH379059    EAL34199.2    JX462969    AGN52762.1    FJ907954    ACQ91145.1    KQ976472    KYM83976.1    CH954177    EDV58092.1    CM000361    CM002910    EDX05202.1    KMY90490.1    CM000157    EDW89676.1    AE014134    AHN54491.1    AY138853    CH480820    EDW54567.1    GDHF01031920    JAI20394.1    CH902620    EDV31384.1    GEZM01077579    GEZM01077578    JAV63266.1    UFQT01000004    SSX17171.1    ADTU01025471    ADTU01025472    ADTU01025473    AXCM01000301    KB632366    ERL93649.1    PYGN01001366    PSN35367.1    GL444277    EFN61058.1    EFN61057.1    KX609519    ARN17926.1    GDHF01012791    JAI39523.1    KQ435724    KOX78300.1    APGK01052905    APGK01052906    KB741216    ENN72534.1    FJ358494    ACO87994.1    KZ288470    PBC25343.1    AY509244    GEZM01072722    JAV65427.1    EFA01264.1    AB987827    BAR72977.1   

Proteomes

UP000005204    UP000218220    UP000053240    UP000053268    UP000037510    UP000027135    + More
UP000235965    UP000009046    UP000015103    UP000075903    UP000002320    UP000076407    UP000075882    UP000075840    UP000075881    UP000007819    UP000075885    UP000007062    UP000008820    UP000075900    UP000183832    UP000007266    UP000092462    UP000192223    UP000069272    UP000075902    UP000078542    UP000000673    UP000053825    UP000037069    UP000092443    UP000092460    UP000075809    UP000078492    UP000092445    UP000095301    UP000092444    UP000078541    UP000008792    UP000092553    UP000009192    UP000078200    UP000268350    UP000001070    UP000095300    UP000091820    UP000008744    UP000007798    UP000001819    UP000078540    UP000192221    UP000008711    UP000000304    UP000002282    UP000000803    UP000001292    UP000007801    UP000076408    UP000005205    UP000075883    UP000030742    UP000245037    UP000000311    UP000079169    UP000053105    UP000019118    UP000242457   

PRIDE

H9IXV2    Q9V399   

Pfam

PF00067   p450        + More
PF06809   NPDC1

Interpro

IPR001128   Cyt_P450        + More
IPR002401   Cyt_P450_E_grp-I       
IPR036396   Cyt_P450_sf       
IPR017972   Cyt_P450_CS       
IPR009635   NPDC1       

SUPFAM

SSF48264   SSF48264       

Gene 3D

1.10.630.10    

ProteinModelPortal

H9IXV2   A0A0K0TQ03   A0A0K0TQ10   A0A0K0TQ19   A0A2H1VQG3   A0A2A4JH06   + More
A0A194QUP5   A0A194PVH1   A0A0L7LAF8   A0A067QWR9   A0A2J7R878   E0VZW4   A0A0A9X2T3   A0A146M3L6   A0A2H4BKC1   R4G5L1   A0A182UYS2   B0WPM4   A0A182XH82   A0A182KYW1   A0A182HR04   A0A182JVQ4   A0A2H8TP40   J9JSS1   A0A182PTI7   Q7Q0Y0   A0A2D1GSE0   Q16MY7   A0A182S4N6   A0A1J1IJF4   A0A139WKQ2   A0A1B0D403   A0A1W4WY09   A0A182G047   A0A182U1F1   A0A0K0LB78   A0A195CB21   W5JGC0   A0A0L7QSC6   A0A0L0BZA4   A0A1A9XVT2   A0A1B0ANX1   A0A151X6S6   A0A195EGE3   A0A1B0A3X4   A0A1I8N5X7   A0A1B0FH35   A0A195FFS5   B4LTE1   A0A0M3QTZ6   A0A1Q1NKZ1   B4KI15   K4JN70   A0A1A9UQZ8   A0A3B0K0V2   B4JAC4   A0A1I8PM70   A0A1A9WNZ4   B4GQM5   B4MZZ0   Q29NL9   R9UDV8   C4PAT7   A0A151I3Z7   A0A1W4VJM7   B3N654   B4Q6Z4   B4NXQ1   X2JA13   Q9V399   B4I210   A0A0K8U0Y7   B3MJP5   A0A182YQN3   A0A1Y1KV34   A0A336LKB5   A0A158NT15   A0A182M230   U4UI40   A0A2P8XTM3   E2AZS9   E2AZS8   A0A3Q0IZM7   A0A1W6L1Q3   A0A0K8VKY7   A0A0M9A6L7   N6SXQ1   C7SHT4   A0A2A3E0Q3   Q6R7M4   A0A1Y1L2U8   D6WFY6   A0A0F7R7X8  

PDB

3T3Z     E-value=1.20377e-47,     Score=480

KEGG

101743283    K07418 cytochrome P450 family 2 subfamily J [EC:1.14.14.1 1.14.14.73 1.14.14.74 1.14.14.75] | (RefSe

PATHWAY

00590    Arachidonic acid metabolism - Bombyx mori (domestic silkworm)
00591    Linoleic acid metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0016705   GO:0005506   GO:0020037   GO:0016021   GO:0004497   GO:0070330   GO:0006805   GO:0006082   GO:0005737   GO:0043231   GO:0008395   GO:0055114   GO:0042738   GO:0016712   GO:0007423   GO:0005789   GO:0006718   GO:0016709   GO:0016805   GO:0005524   GO:0048013   GO:0005003   GO:0005887   GO:0007169  

Subcellular location

Endoplasmic reticulum membrane  
Microsome membrane  

SignalP

Position:   1 - 23,         Likelihood:  0.714576

 

 

Length:

498

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

28.46438

Exp number, first 60 AAs:

15.69121

Total prob of N-in:

0.70446

POSSIBLE N-term signal

sequence

inside

1  -  1

TMhelix

2  -  21

outside

22  -  498

 

 

Pi

4.07242

Theta

6.624764

Tajima's D

-1.01997

CLR

0

CSRT

0.125043747812609

Interpretation

Uncertain